what is use of acetonitrile compare to methanol in RP-HPLC
Method devlopment
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Answer Posted / balaji
the uv cut oof value of methanol is 210 nm so we cant use
methanol near about lambda max of 200 nm. so prefer to ACN
& ACN is highly polar as compare to Methanol So provide
Better resolution for many compound and it has property to
form hydrogen bond so provide better selectivity.
Methanol viscosity more than the ACN. so ACN easly flow
through column
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