Answer / bujji kanchi

1)inject same concentration of impurity and active

Ratio of impurity area and active area

correct the concentrations.


Best practice is

Inject LOQ to 150% of specification around 5 levels

and calculate the slope of impurity and slope of active

take the slpe ratio.



Regards,
Bujji Kanchi.

Answer / javed

RRF Can be calculated by comparing the area of standard with
area of impurity x conc of imp by conc of std x 100.

Is it necessary to inject three replicate for accuracy in AMV.

1 Answers   Amoli Organics,

---

In the test for control of absorbance in UV calibration why do we use only potassium dichromate and what is purpose of taking a specified amount of 57.0-63.0mg?

1 Answers   Reddy Labs,

---

why has inject 5 injection in assay

2 Answers   Lupin,

---

what is mean by UOD?

2 Answers  

---

how to set sample and standard concentration in RS method?

0 Answers  

---

why we are using 6 dosage units in dissolution, and why we are using 6 tablets in disintegation test.is there any scientific reason beyond 6?

1 Answers   ABC, Alembic, IPCA,

---

In Analytical method development related substance method when did follow w/w method when did follow area normalisation method

5 Answers  

---

Can any one tell what is RECALL OF PROCEDURES is

1 Answers   Macleods,

---

identification is for unknown? qualification for known? reporting for LOQ?

0 Answers  

---

why are measure gas flow " ml " in Gas chromatography

0 Answers  

---

What is the limit of minimum area in in hplc RS anlysis

1 Answers   Lupin,

---

which pharmacopeoia mentions that caffiene is used as a standard for HPLC calibration? which other standardscan be used for the same?

1 Answers   Hetero, Mylan,

---