HOW RRF IS CALCULATED IN HPLC
- 2 Answers
- 10723 Views
- I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / bujji kanchi
1)inject same concentration of impurity and active
Ratio of impurity area and active area
correct the concentrations.
Best practice is
Inject LOQ to 150% of specification around 5 levels
and calculate the slope of impurity and slope of active
take the slpe ratio.
Regards,
Bujji Kanchi.
| Is This Answer Correct ? | 8 Yes | 0 No |
Answer / javed
RRF Can be calculated by comparing the area of standard with
area of impurity x conc of imp by conc of std x 100.
| Is This Answer Correct ? | 9 Yes | 8 No |
What is the requirement for brazil in the analytical method vslidation comparr to ich?
hii, i'm had a sample of 1L that gone through extraction & evaporation. i used 0.5ml of methanol for reconstitution before injecting the sample to HPLC. i made a calibration curve using a standard solution but now i'm confused about how to display the concentration i got from the sample. for ex. i got 0.2ug/ml how i'm gonna explane this is for the 1L ? please help .. i'll really appreciate it thanks
In Related Substances Test how we can reside the limit of impurity ?
how require to calculate mass balance in related sub?
How can the GC or HPLC method is selected to determine the impurity profile in drug product?
when we use capillary and packed columns in gc
Why monograph of hplc coming upperside of drift but in IR monograph coming in drift side?
what is DIPE limit in residual solvent?
What do you mean by potency in Analytical industry
Hi sir if any product monograph not given known impurities (may be 5 impurities) specifications then how we require to proceed for inhouse formulation?
What is the b/W dead volume and dwell volume ?
In Dissolution Test why limit is define Q+5% what is the role of +5%.
Organic Chemistry 
