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HOW RRF IS CALCULATED IN HPLC

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HOW RRF IS CALCULATED IN HPLC..

Answer / bujji kanchi

1)inject same concentration of impurity and active

Ratio of impurity area and active area

correct the concentrations.


Best practice is

Inject LOQ to 150% of specification around 5 levels

and calculate the slope of impurity and slope of active

take the slpe ratio.



Regards,
Bujji Kanchi.

Is This Answer Correct ?    8 Yes 0 No

HOW RRF IS CALCULATED IN HPLC..

Answer / javed

RRF Can be calculated by comparing the area of standard with
area of impurity x conc of imp by conc of std x 100.

Is This Answer Correct ?    9 Yes 8 No

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