in hplc calibration we are using 0.05% acetone why we are
using that one and what happen when its conc increases or
decreases

Answers were Sorted based on User's Feedback



in hplc calibration we are using 0.05% acetone why we are using that one and what happen when its ..

Answer / ichinnakamisetti

acetone gives maximum absorbance at 260nm

Is This Answer Correct ?    8 Yes 4 No

in hplc calibration we are using 0.05% acetone why we are using that one and what happen when its ..

Answer / manoj k kasar

It is not compulsary to use acetone, if we use any compound(which is soluble in particular solvent) having maximum absorbsnce at particular wavength,then also we got the stepwise calibration like acetone gradiant stepwise calibration in hplc.

If ur time program is correct to obtain stepwise calibration of acetone then nothing will happens if u increase or decrease the conc. of acetone.

Is This Answer Correct ?    10 Yes 9 No

in hplc calibration we are using 0.05% acetone why we are using that one and what happen when its ..

Answer / a.iyyappan

0.05% Acetone is used in HPLC GPV(Gradient performance verification)test, since its heigher level of absorption.
Not only Acetone, caffeine also recommended for this test.
0.05% Acetone and Caffeine are recommended by HPLC manufacture(Shimadzu).

Is This Answer Correct ?    3 Yes 3 No

in hplc calibration we are using 0.05% acetone why we are using that one and what happen when its ..

Answer / ramu

acetone uv range 230 that's y

Is This Answer Correct ?    3 Yes 12 No

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