in hplc calibration we are using 0.05% acetone why we are using that one and w

in hplc calibration we are using 0.05% acetone why we are
using that one and what happen when its conc increases or
decreases

Question Posted / jayapradhagreddy

4 Answers
32308 Views
Aurobindo, Cadila, Martin, I also Faced
E-Mail Answers

Answers were Sorted based on User's Feedback

in hplc calibration we are using 0.05% acetone why we are using that one and what happen when its ..

Answer / ichinnakamisetti

acetone gives maximum absorbance at 260nm

Is This Answer Correct ?

8 Yes

4 No

in hplc calibration we are using 0.05% acetone why we are using that one and what happen when its ..

Answer / manoj k kasar

It is not compulsary to use acetone, if we use any compound(which is soluble in particular solvent) having maximum absorbsnce at particular wavength,then also we got the stepwise calibration like acetone gradiant stepwise calibration in hplc.

If ur time program is correct to obtain stepwise calibration of acetone then nothing will happens if u increase or decrease the conc. of acetone.

Is This Answer Correct ?

10 Yes

9 No

in hplc calibration we are using 0.05% acetone why we are using that one and what happen when its ..

Answer / a.iyyappan

0.05% Acetone is used in HPLC GPV(Gradient performance verification)test, since its heigher level of absorption.
Not only Acetone, caffeine also recommended for this test.
0.05% Acetone and Caffeine are recommended by HPLC manufacture(Shimadzu).

Is This Answer Correct ?

3 Yes

4 No

in hplc calibration we are using 0.05% acetone why we are using that one and what happen when its ..

Answer / ramu

acetone uv range 230 that's y

Is This Answer Correct ?

3 Yes

12 No

Post New Answer

More Analytical Chemistry Interview Questions

we are performed SOR for a particular product the limit is -6 to -10.We face particular bathes it is not meeting the specification.we performed diffrent instrument diffrent analyst we are getting diffrent diffrent results.solvent is dimethyl sulfoxide what could be the reasion

0 Answers Cipla,

cefoperozone and sulbactam inj. hplc test method

0 Answers Graviti Pharmaceuticals,

why do we use around only 60 mg of K2cr2o7 in the UV calibration -control of absorbance test?

3 Answers Reddy Labs,

what is batch no. of prednisole tablet.

8 Answers Dr Reddys,

What is the difference between GSC & GLC?

3 Answers

How require to perform linearity as per ANVISA? What are the acceptance criteria?

0 Answers

What is Endcapped Hplc column