Interested to Buy Any Domain ? << Click Here >> for more details...
in hplc calibration we are using 0.05% acetone why we are
using that one and what happen when its conc increases or
decreases
- 4 Answers
- 32107 Views
- Aurobindo, Cadila, Martin, I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
acetone gives maximum absorbance at 260nm
| Is This Answer Correct ? | 8 Yes | 4 No |
Answer / manoj k kasar
It is not compulsary to use acetone, if we use any compound(which is soluble in particular solvent) having maximum absorbsnce at particular wavength,then also we got the stepwise calibration like acetone gradiant stepwise calibration in hplc.
If ur time program is correct to obtain stepwise calibration of acetone then nothing will happens if u increase or decrease the conc. of acetone.
| Is This Answer Correct ? | 10 Yes | 9 No |
Answer / a.iyyappan
0.05% Acetone is used in HPLC GPV(Gradient performance verification)test, since its heigher level of absorption.
Not only Acetone, caffeine also recommended for this test.
0.05% Acetone and Caffeine are recommended by HPLC manufacture(Shimadzu).
| Is This Answer Correct ? | 3 Yes | 4 No |
Why 1800 ml of dissolution media used In Carbamazepine ER USP Tablets But other drug only 900 ml Media Used ?
How to calibration of the uv spectroscopy and its test?
How can we Qualify a unknown impurity by HPLC
In UV we measure absorbance , how it is happend ? explain me
which brands GC coloumn available in market
if peak get problamatic then what require to do?
in which situation require to change rs specification?
How to calculate coreletion coefficient
Why Urasil and Caffeine use for HPLC wavelength accuracy calibration. which is most suitable, Why? other than these which can be used?
How to fix the sample concentration in related substances development.
3 Answers Novartis, Watson Pharmaceuticals,
What is impurities in pharma analysis?
When we calculate Impurity against impurity std.solution and when we calculate impurity against diluted standard with applying RRF?
Organic Chemistry 
