in hplc calibration we are using 0.05% acetone why we are
using that one and what happen when its conc increases or
decreases
Answer Posted / ichinnakamisetti
acetone gives maximum absorbance at 260nm
Is This Answer Correct ? | 8 Yes | 4 No |
Post New Answer View All Answers
function of detecter in hplc ,gc and spectroscopy? function of carrier gas in gc?
if you have given a blank water how you analyse by general analytical techniques?
for heavy metal test lead used in which form pure form or any other form for preparation of lead standard sol
Tell me something about Vitamin A test method by HPLC
we can use expired sample for validation and analyst qualification?
what is the principle of UV Vis spectroscopy, AAS, ICP OES,ICPAES, ICP-MS and FTIR
how decide the clining method and cleaning method validation require for this perticular products?
How require to develop GC method? how to select diluent, GAS, column selection and other chromatographic conditions?
what is turbidimetric titration?Give 2 examples.
please explian me about area normalisation method,diluted standard method,impurity vs impurity and which one has to be selected in method development
what is the importance of peak purity in HPLC and how we can calculate through manul(not software)?
How to determine water content of bis tetrazole amine mono ammonium salt
If change in specification which parameter require to do for validation? if change in chromtographic condition then which parameter? if api change then which parameter? if change composition then which parameter? if old method not work out then whicj parameter? if additional one impurity added then which parameter of validation require to do on above each conditions? elaborate separately
In HPLC calibration, caffeine is used as primary standard for wave length calibration due to caffeine is having dual maxima at 273 & 205 nm and one minima at 245 nm. Any body can give reference of these details from any pharmacopeia (with chapter no.) or any other authentic guideline?
If we have 5 strength which is not dose proportinate and excipients also diffrent in each strength then how we can proceed for Force degradation? and excipient are same but not dose propotinate the how FD?