How we develop a method by HPLC for unknown compound,if we got three peaks with same response then how we can find out which one is our desired compound's peak without taking help of LC MS.
Answer Posted / dinesh solanki
If it is a assay method then inject std in hplc system and compare the chromatogram and identified the interested peak from them.
Same RC method inject std and identified the interested peak and other is may be impurities or contamination of sample.
| Is This Answer Correct ? | 2 Yes | 1 No |
Post New Answer View All Answers
Why sodium hydroxide used for maintain pH of phosphate buffer
what is difference between UV - VISIBLE MODEL NO like 1600,1601,1700 etc ? plz explain me
Why acetonitrile and water are used as extraction solvent when analysis melamine? I thought they are miscible and won't be able to separate...
which batch require to use for analytical method validation?
Is it nessesary all multimedia dissolution require descriminatory?
What is shaking level in GC?
What is mean by PDR?
Why are use silicon oil mr?
Why potassium dichromate is used in calibration of uv?
what is mean by nitrosamine impurities?
analytical method validation require to with respect to release specification or shelf life specification?
what is the principle involved in turbidometry and nephelometry ?
WHY pKa value is more important than pH value of the mobile phase in HPLC?
Tell me about analytical method validation in QC
For limit test of heavy metals in BP, Method C require that the substance is ignited at a temperature not exceeding 800 °C. Why confines such the temperature?