In Karl Fischer titration,What is the situation if the
material tested is only soluble in water,and not soluble in
the common solvents used such as methanol ?? [e.g. Iron III
Hydroxide polymaltose]
Answer / sana
We go to thermoprep it is a different technique apart from kf
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In my analysis by HPLC,In sample main peak and placebo peak are eluting with shorter resolution ( almost look like doublet). how can i get max resolution ? Is changing gradient method is usefull?
In HPLC Chromatographic separation Resolution equation is Rs = 1.18 (tR2 –tR1)/wh1+Wh2, Where, tR2 and tR1 is run time of eluent ratios of the distances from the point of application to the centers of the spots and the distance travelled by the solvent front from the point of application (retardation factor), and Wh1and Wh2 , is peak width of at half height. What is 1.18 ?
How do we fix the sample concentaryion in hplc method development. What is the basis?
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Why the same type of columns showing different RT for a single product?
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