Interested to Buy Any Domain ? << Click Here >> for more details...
In the test for control of absorbance in UV calibration why
do we use only potassium dichromate and what is purpose of
taking a specified amount of 57.0-63.0mg?
- 8 Answers
- 59194 Views
- Aurobindo, Cipla, FDC, I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / bhagya lakshmi.k
It will show max absorbance in Ultraviolet region (200-
400nm) (i.e) at 235,257,313,350 nm
| Is This Answer Correct ? | 53 Yes | 4 No |
Answer / sunil.m.kulkarni ( avon/arch p
Potassium Dichromate - UV Absorbance and Linearity
Product Description:
The use of potassium dichromate solvated in dilute
perchloric acid or suplhuric acid is an established and
well recognised method for the validation of the absorbance
scale and linearity of a spectrophotometer in the UV
region.
When prepared in 0.001M perchloric /Suplhuric acid,
potassium dichromate solution gives a spectral scan
containing characteristic peaks at 257 nm and 350 nm, and
troughs at 235 nm and 313 nm. Within the concentration
range 20-100 mg/l, if the absorbance scale of a narrow SBW
(< 2 nm) spectrophotometer is linear, the apparent
absorbances of a series of concentrations will be a linear
function of concentration.
Note: Primary standard ( Extra pure potassium dichromte
after drying to be used for calibration ) 57-63mg in -
1000ml concentration is perfect concentration for achiving
perfect charecteristic peak and throughs
| Is This Answer Correct ? | 36 Yes | 5 No |
Answer / sagadevan
becoz of photochemical stability,so on prolonged exposure to uv light the absorbance own not change,
| Is This Answer Correct ? | 18 Yes | 4 No |
Answer / devidas
it show the maxima at 235nm,257nm,313nm, and 350nm in UV
region. and it consider as a primary standard which is high
purity
| Is This Answer Correct ? | 18 Yes | 7 No |
Answer / vivek
we are use potassium dichromate for the verification of spectrophotometer because it show maximum absorbtion in uv region...
| Is This Answer Correct ? | 12 Yes | 5 No |
Answer / srinathsagar
UV range is 190-400nm so in this range potassium dichromate with sulfuric acid gives characteristic peaks(At 235,257,313 and 350nm)
| Is This Answer Correct ? | 4 Yes | 1 No |
because it covers uv to visible region i.e. 235nm,257nm,313nm,350nm
| Is This Answer Correct ? | 6 Yes | 4 No |
what is the analytical procedure for COD and BoD
In method developement or in any analysis if we use small length column for analysis what happen then ? why ?
how to prepare 5 normality of h2so4 solution
What is peak purity and response factor in chromatographic analysis? Why we required to find out response factor?
Column selection in HPLC method development?
What is Endcapped Hplc column
Why sometime potassium ortho phosphate mix with acetone use for sample preparation for hplc
What is the SOP for SOP
what is diffrence between shelf life and expiry date?
What are the parameters for the method development of Gas Chromatography and GC-HS?
Whatn is HPLC
1 Answers HPLC, Jubilant, Orchid,
wat is reverse phase in hplc
Organic Chemistry 
