What is peak purity and response factor in chromatographic analysis? Why we required to find out response factor?
Answers were Sorted based on User's Feedback
Answer / venkata naveen talluri
Peak purity=It indicates whether the peak is single or co-eluting with any other analyte. If peak purity is passing it indicates it is single peak. based on the Noise and co-eluting peaks the peak purity depends.
Response factor/Relative response factor/RRF=the response of an impurity withrespect to main peak at a particular nanometer.All impurities will not have equal responses with API. RRF needs to be established because impurities are very costly and each time of analysis it is difficult to be used. So establish an RRF and use the RRF for calculation in repoting the impurity value
Is This Answer Correct ? | 11 Yes | 0 No |
the peak purity test is chromatographic analyte peak is not attributable to more then one component. this can be determined by PDA detector/LC MS.
Is This Answer Correct ? | 2 Yes | 0 No |
what is the procedure for cleaning of lenses of hatr accesory of ftir instrument?
why we are using hexane in calibration of number of drop per mL
formula for %RSD.
what is the conductivity of hplc grade water?
Why void volume(time in seconds) 1 in hplc system suitability
what is the Bracketing & Matrixing.
In lcms analysis some times we get sodium and potassium adducts. if the compound is havine sodium ions how can we confirm wether sodium is from sample or it is a adduct?
2 Answers Teva Pharmaceuticals,
key components in hplc?
what is difference in ods I ods II is this is realy from sorbent / packing characteristics or from other means
IF HPLC CALIBRATION FAILED THEN WHAT YOU DO
if the purity is 99.9%,is need to do assay?
How to Calibrate NMR instrument ?