Interested to Buy Any Domain ? << Click Here >> for more details...
In the test for control of absorbance in UV calibration why
do we use only potassium dichromate and what is purpose of
taking a specified amount of 57.0-63.0mg?
- 8 Answers
- 58924 Views
- Aurobindo, Cipla, FDC, I also Faced
- E-Mail Answers
Answer Posted / sunil.m.kulkarni ( avon/arch p
Potassium Dichromate - UV Absorbance and Linearity
Product Description:
The use of potassium dichromate solvated in dilute
perchloric acid or suplhuric acid is an established and
well recognised method for the validation of the absorbance
scale and linearity of a spectrophotometer in the UV
region.
When prepared in 0.001M perchloric /Suplhuric acid,
potassium dichromate solution gives a spectral scan
containing characteristic peaks at 257 nm and 350 nm, and
troughs at 235 nm and 313 nm. Within the concentration
range 20-100 mg/l, if the absorbance scale of a narrow SBW
(< 2 nm) spectrophotometer is linear, the apparent
absorbances of a series of concentrations will be a linear
function of concentration.
Note: Primary standard ( Extra pure potassium dichromte
after drying to be used for calibration ) 57-63mg in -
1000ml concentration is perfect concentration for achiving
perfect charecteristic peak and throughs
| Is This Answer Correct ? | 36 Yes | 5 No |
Post New Answer View All Answers
What are modes of quantification in related substances
in each media we require to use SLS? how to proceed?
If vendor having more imp than monograph then how to proceed? and how to set spec?
how to decide for one product require water content or LOD?
Why sometime potassium ortho phosphate mix with acetone use for sample preparation for hplc
If we do accuracy at same concentration at which linearity planned,what is the need to do linearity separately?
If inhouse hplc related substance method is completly diffrent from Usp for finished proďuct with diffrent impurities then how require to prove method equivalecy?
In HPLC Calibration, On which basis RSD Limit of noise test is fixed (NMT 33.0 % )
WHAT IS THE PURPOSE WE USE HCL, CHOPPER, BURNER, MONOCHROMATOR in AAS? PLEASE EXPLAIN BRIEFLY
How to set specification of assay, fisdolution and related substances?
how require to set assay concentration for standard and sample?
why xterra column require to use at higher ph?
How require to develop GC method? how to select diluent, GAS, column selection and other chromatographic conditions?
In BP2013, Loperamid HCl monograph. Assay by titration with 0.1N sodium hydroxide using hydrocloric acid 0.01N and reading the volume added between the 2 points of inflexion. I have a question that if the diluent solvent is ethanol is certainly consumed a amount of volume of titrant, so this volume must be eliminated on the result calculation or not apart from first point which is subtracted above.
which one is better separation in high pressure liquide chromatogram orlowpressure chromato gram in hplc
