Difference between c18
- 6 Answers
- 50601 Views
- Apothecon, Dr Reddys, Intas, IPQA, SUN, Torrent Pharma, I also Faced
- E-Mail Answers
Answer Posted / jeevithan
Which type of column should i use to check the purity of high molecular weight protein using HPLC reverse phase column chromatography? Hi everyone. I wanna to check the purity of high molecular weight protein (collagen) with MW of ~130 kDa using a HPLC. I know C18 & C8 reverse-phase column (particle size 3 & 5 µm) can be used to separate low molecular weight protein and peptides. Anyone can suggest me the "specification of particle size" of HPLC column for high MW protein?
Which type of column should i use to check the purity of high molecular weight protein using HPLC reverse phase column chromatography? - ResearchGate. Available from: https://www.researchgate.net/post/Which_type_of_column_should_i_use_to_check_the_purity_of_high_molecular_weight_protein_using_HPLC_reverse_phase_column_chromatography [accessed Jun 27, 2015].
| Is This Answer Correct ? | 3 Yes | 0 No |
Post New Answer View All Answers
if you have given one product then how you determine the impurity in that?
in which situation ion pair agent require to use?
1)What's the meaning of Absorption,give a example. 2)What's the meaning of Adsorption,give a example. 2)what is the difference between Absorption and Adsorption.
What is the formula to determine the concentration of M of a solution given the % transmittance? Use %T = 43.7 as an example.
In HPLC (Waters) software Empower1,2 &3 what is the updation for each version
If inhouse hplc related substance method is completly diffrent from Usp for finished proďuct with diffrent impurities then how require to prove method equivalecy?
In performance Check of GC Why Hexadecane Peak is Considered
For standardization of volumetric solutions, acceptance limit for concentration is considered as 10% of nominal concentration and %RSD as NMT 0.2% (for appropriate no. of replicates). But , if %RSD has not met, what should be further action?
What is control room temperature and which guide line says?
What is importance of pH in the Heavy metal test and How it effect on the test results?
Why potassium dichromate is used in calibration of uv?
if peak get problamatic then what require to do?
Why we used in n-butyl acetate water content terminology while in ethyl acetate we used moisture content terminology?
How to decide assay range for non pharmacopeial API analysis by HPLC? Could you give me any reference for same e.g Guidelines or Paper publications?
what is mean by 40 in the dissolution basket mesh size?
