Which amount of DMEM and Glutamine should be commaneded ?



Which amount of DMEM and Glutamine should be commaneded ?..

Answer / Lal Singh Garbyal

The amount of DMEM and L-glutamine required for a tissue culture depends on the specific cell type, growth requirements, and experimental conditions. Generally, 10% FBS (Fetal Bovine Serum) is added to 90% DMEM for maintaining most adherent cell lines. For glutamine, it is often supplemented at a concentration of 2 mM.

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