Which separates the protein antigen mixture in Immunoblotting technique?1 3416
How are free energy, equilibrium and spontaneity related to each other?
Hi, I am venkat. I am done with M.Sc Medical Biochemistry in the year 2007. I got rejected 3 times at US consulate, and the reason they say is why are you going agian for PG when you have already done PG, and they are not ready to get convinced only on this issue.
Calculate the molar extinction coefficient of a solution containing 5 *10-4 g litre-1 of a biomolecule, molecular weight 275 g mol-1, and absorbance 0.75 in a 1.2 cm cuvette.
What are hydrophobicity scales, and how are they used?
What are species grow in waitland?
How does hplc differ from normal column chromatography, and what are its advantages?
why ph is not more than 14?
I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line
Describe the structure of a peptide giving the sequence.
why life span of RBC IS GREATER THEN THE WBC?
What do you like LEAST & MOST about your current position?
i have done M.SC. in biochemistry from india. as i have experience of pharmaceuticals in quality assurance department of 4 year.now i come to know that my degree is valid in uk.i got my comparision letter of degree from uknaric. but i m confuse that what is the next step to do for getiing job in lab? am i eligible to get directly job or as trainee than job ? or to register in ibms or what? pls tell me
how I cell disease is caused?
When it functions as a "second messenger", cAMP a) acts outside the cell to influence cellular processes. b) acts "second in importance" to AMP. c) activates all cytosolic protein kinases. d) activates the cAMP-dependent protein kinase.
which fat soluble vitamin has co-enzymic function?