What is the reason of RT change in new column v/s old column in HPLC
Answer Posted / dipty
Because the old column might not be washed properly so the stationary phase which is not used up that is if the column is new it has more chance to hold up the drug.
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My question about gas chromatography sulfur chemiluminsecence detector. I test unknown sample gas by GC-SCD (calibrated ) and the result of *H2S is 279 PPM , *but when I test the same sample with the GC-TCD (calibrated ) the value of *H2S is 2500 PPM . I'd like to inform you that both GCs are calibrated and have very good operation conditions with stable parameters . the question is if the sample gas with higher H2S over detection limits of SCD detector (1000 ppm). why the result it 279 ppm Best regards
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