What is difference between silica used in
TLC and HPLC column
Answers were Sorted based on User's Feedback
Answer / sanjay
MAjor difference in Purity and Particle size of Silica in
TLC and HPLC
| Is This Answer Correct ? | 13 Yes | 1 No |
Answer / durga malleswar
the difference in tlc the stationary phase is polar funtioned silica and in hplc we use stationary phase with non polar functioned silica only the difference is the functional groups of the silica changed
| Is This Answer Correct ? | 10 Yes | 0 No |
Answer / vijaya kumar thota
Tlc is called thin layer chromatography. The simple technique works on the principle of adsorption, where as HPLC is a sophisticated technique works on partition principle.
| Is This Answer Correct ? | 25 Yes | 22 No |
Answer / mabood alam
thin layer cromatigrophy and high coloum cromatigrophy used
in cilka
| Is This Answer Correct ? | 4 Yes | 12 No |
Why we show ammonia vapours to tlc plate?to which compounds?
what are the important specifications for a Column ?
Why do we need GC instead of HPLC? or Why do we need HPLC instead of GC?
What is primary & Secondary Standard ? Give Example
1)why use benzophenon,uracil in hplc calibration. 2)why use sucrose in polarimeter calibration. 3)what is S/N ratio, noise and drift in hplc 4)how we can find lod and loq in method validation
what is the balance drift?
When ever we perform system suitability,inject the corresponding standard 6 times why? is thair any reson
whate is difference between calibration and validation
11 Answers Alkem Labs, Cipla, Ranbaxy, Torrent Pharma, Zydus Cadila,
Column selection in HPLC method development?
if the flow rate is increases then any changes in retention time in hplc
How we can analysed unknown sample by hplc....
why equlirium conatant of water have 14 value