Why the same type of columns showing different RT for a single
product?
- 6 Answers
- 14451 Views
- I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / amol
because , when manufacturing of column is done different
parameter is affected which is given below , due to this
each column is unique tho it is same type.
column parameter
column pressure
parical size
carbon density
dead voulume of the column
end fitting
end capping of the column.
silica used.
Hplc System Parameter
length of tubing.
internal dia. of tube.
void volume of system.
pump working.
flow rate.
end fittings.
ETC....
| Is This Answer Correct ? | 20 Yes | 2 No |
Answer / satheesh kumar
In HPLC same type of columns means say C18,there are lot of
brands C18 columns available in market namely
inertsil,Symmetry,X-Terra,phenomenex gemini,Zorbax etc.,
Each columns are not similar in packing and carbon
load.column chemistries will vary with one another.
Hence RT shift observed in same types of columns.
| Is This Answer Correct ? | 3 Yes | 0 No |
Answer / reet
following factors might be the reason:
1. Column ageing
2. how the column was preserved, or what mobile phase was
used before the present experiment
3. sample preparation might be the cause. sample may be non-
homogeneous
4. Slight variation in thepH may also affect the variation
in RT.
5. Other parameters like,temperature can also be one of the
reason.
6. last but an imporatant factor might be some air bubble
trapped in the system.
| Is This Answer Correct ? | 3 Yes | 2 No |
Answer / ali
The difference in RT is usually caused by column age and how
it was conditioned before the analysis.
| Is This Answer Correct ? | 4 Yes | 8 No |
Answer / ravi.a
it is depend on used mobile phase is one of the reason if u
get peaks area are same if not get same areas that column
is dameged due to in colun stationary phaes coming ot side
| Is This Answer Correct ? | 2 Yes | 6 No |
how require to calculate mass balance in related sub?
How to set specification of assay, fisdolution and related substances?
Which buffer is used for LC/MS mobile phase & at What Conc.
In GC Why does retention time decrease when temperature increases? Also how does a change in temperature effect selectivity?
How much sample concentration should i inject against diluted standard in related substances for drug product in hplc analysis
hii, i'm had a sample of 1L that gone through extraction & evaporation. i used 0.5ml of methanol for reconstitution before injecting the sample to HPLC. i made a calibration curve using a standard solution but now i'm confused about how to display the concentration i got from the sample. for ex. i got 0.2ug/ml how i'm gonna explane this is for the 1L ? please help .. i'll really appreciate it thanks
what is the definition of limit of stray light in UV calibration
why holium oxide prepared in 10% HCLO4 SOLUTION
why we use actone for Gpv test in hplc calibration
What is the difference between high pressure gradient and low pressure gradient in hplc?
How to prepare ppm solution of Nacl form its salt?
What is diffrence between residue on ignition and sulphated ash?
37 Answers Aurobindo, Cadila, Dr Reddys, Mylan, Panas Pharmaceuticals, Piramal Healthcare, Remidex, Zydus Cadila,
Organic Chemistry 
