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how can we determine RRF value in HPLC FOR CALCULATION OF
IMPURITY ?
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Answer Posted / balaji
RRF-Relative responce factor with respect to analyte(standard)
why its required- due to impurity cost,availability and time
consumption of analysis etc we can not go for everytime
impurity by impurity calculation every time
so,that finding a factor for impurities with respect to
standard.by preparing few levels of(3+ levels)standard and
impurities concentrations(can prepare between +/- 50% of
specification level of known impurities and for standard
between +/- 50% of specification level of unknown
impurities).inject the above prepared solution in to
hplc,calculate slope for impurities and standard by using
respective concentrations and areas.
RRF=SLOPE OF IMPURITIES/SLOPE OF STANDARD
| Is This Answer Correct ? | 55 Yes | 7 No |
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