Answer / tanu bhardwaj

when we prepare a solution for UV-vis spectroscopy, we prepare it in a solvent, to remove the absorption effects due to solvent, we keep that solvent into blank, so that when the two rays coming out of two cuvettes are subtracted, then we get only the absorption due to solute, not due to solvent. if we prepare sample in water, then water is used as blank.

Answer / pnreddy

uv 200nm to 400nm
visible 400nm to 800 nm
because solvent is water

Answer / naveen kumar

UV is not applicable for the high concentration solutions means 0.01M Nd it is not applicable for suspensions emulsions coagulated products Nd florescence substances

Answer / kamil hassan

limitation of uv visible spectrometer from 200 nm to 800 nm range. 200 to 400nm uv wavelength & 400 to 800 in visible Wavelength '
water we used as a solver in spectrometer because its absorb on only 165 nm (vacuum uv range) so why we used deionized water as a Blank.

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