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What is the Column Endcaping in HPLC ?
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Answer / rajkumar
In columns Stationary phase is Organic phase is coated over
the Silica surface. But during manufacturing it is not
possiable that all active sites(Silica surface)are coated
with organic phase there will be some vacant areas. In that
areas it is possiable to coat with Long chain Organic phase.
so small chain such CH3 is coated in that areas.
| Is This Answer Correct ? | 17 Yes | 1 No |
Answer / suresh palacharla
A reversed-phase HPLC column that is end-capped has gone
through a secondary bonding step to cover unreacted silanols
on the silica surface.
| Is This Answer Correct ? | 10 Yes | 0 No |
Answer / ganesh bhavar
for endcapping in reversed phase column use Trimethylamine
means it protect the OH-group silanol group
| Is This Answer Correct ? | 2 Yes | 4 No |
why we are using buffer solutionas as a mobile phase in hplc.
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