Answer / rajkumar

In columns Stationary phase is Organic phase is coated over
the Silica surface. But during manufacturing it is not
possiable that all active sites(Silica surface)are coated
with organic phase there will be some vacant areas. In that
areas it is possiable to coat with Long chain Organic phase.
so small chain such CH3 is coated in that areas.

Answer / suresh palacharla

A reversed-phase HPLC column that is end-capped has gone
through a secondary bonding step to cover unreacted silanols
on the silica surface.

Answer / ganesh bhavar

for endcapping in reversed phase column use Trimethylamine
means it protect the OH-group silanol group

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