How to select buffer for HPLC mobile phase




Answers were Sorted based on User's Feedback



Answer / saravanan

According to the pKa value of the compound

Is This Answer Correct ?    47 Yes 4 No

Answer / m

Accordingly compound PKa value

Is This Answer Correct ?    43 Yes 6 No

Answer / kaushlendra singh

pH of buffer should be + or minus 1 away from pK of
compound and near to the pK value of buffer or salt use in
mobile phase preparation.

Is This Answer Correct ?    5 Yes 2 No




Answer / qamaruddin

According to the pKa value of the compound

Is This Answer Correct ?    1 Yes 0 No

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