Answer / aroh shrivastava

Depending on the source, the protein has to be brought into
solution by breaking the tissue or cells containing it.
There are several methods to achieve this: Repeated
freezing and thawing, sonication, homogenization by high
pressure, clarification via cellulose-based depth filters,
or permeabilization by organic solvents. The method of
choice depends on how fragile the protein is and how sturdy
the cells are. After this extraction process soluble
proteins will be in the solvent, and can be separated from
cell membranes, DNA etc. by centrifugation. The extraction
process also extracts proteases, which will start digesting
the proteins in the solution. If the protein is sensitive
to proteolysis, it is usually desirable to proceed quickly,
and keep the extract cooled, to slow down proteolysis

Answer / guest

ZEOLITE METGHOD

QUANTUM BIOLOGY - EXAMPLE 33.5 : (a) In the measurement of the number of photons received by a plant during photosynthesis, quantum meter is used. At 8 AM, 11 AM, 12 PM, 2 PM and 4 PM of the same day, the measured readings of the meter are 10, 70, 60, 120 and 120 units. Find the mode, median and min of the readings of the quantum meter. (b) Microtubule is used to carry information in a cell. If the cross-sectional area of the microtubule has a diameter of 25 nanometers, find the volume of the microtubule of 1 nanometer in height. State the assumption of calculation.

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