Answer / aroh shrivastava

Depending on the source, the protein has to be brought into
solution by breaking the tissue or cells containing it.
There are several methods to achieve this: Repeated
freezing and thawing, sonication, homogenization by high
pressure, clarification via cellulose-based depth filters,
or permeabilization by organic solvents. The method of
choice depends on how fragile the protein is and how sturdy
the cells are. After this extraction process soluble
proteins will be in the solvent, and can be separated from
cell membranes, DNA etc. by centrifugation. The extraction
process also extracts proteases, which will start digesting
the proteins in the solution. If the protein is sensitive
to proteolysis, it is usually desirable to proceed quickly,
and keep the extract cooled, to slow down proteolysis

Answer / guest

ZEOLITE METGHOD

ENGINEERING NUMERICAL METHODS - EXAMPLE 19.3 : There are 2 simultaneous equations : (A1) x + (B1) y = D1 and (A2) x + (B2) y = D2. (a) By using Excel program, find the values of x and y when A1 = 80, A2 = 150, B1 = 52, B2 = 100, D1 = 3.5 and D2 = 2.3. (b) Write the expression of Excel in the form of =MMULT(MINVERSE(W:X),Y:Z) in order to get the values of x and y. W, X, Y and Z may be A1, A2, B1, B2, D1 and D2.

1 Answers  

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Dielectric materials in static fields

0 Answers   HCL,

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Which type of drier is used for drying wet material?

3 Answers  

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What is the process of Scrubber ?

3 Answers  

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sir i am senthil B.Tech Chemical Engineer which book is refer for objective type questions?

2 Answers   BARC, GATE,

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BIOCHEMICAL ENGINEERING INSTRUMENTATION - EXAMPLE 29.5 : The following formula is used as a reference : (analyte signal) / (internal standard signal) = (f-factor) x (concentration of analyte) / (concentration of internal standard). A solution containing 3 mM of analyte and 4 mM of internal standard gave peak signals of 2 and 3 mamps respectively. Another similar solution containing 2 mM of analyte and 1 mM of internal standard gave peak signals of 1 and 4 mamps respectively. Find the average f-factor.

1 Answers  

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I would like to know how to size a partial combustion reactor processing methane and oxygen, as a function of the flowrate and of the pressure.

0 Answers  

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Question 64 – According to a heuristic of chemical engineering plant design, assume a pressure difference dP = 4 psi for each 10-ft rise in elevation. A pump is needed to pump liquid from a storage tank at a lower elevation to a heating tank at a higher elevation with the elevation difference of 60 ft. (a) Find the pressure loss due to such elevation. (b) If the required minimum inlet pressure to the heating tank is 9 psi, with 1 control valve is installed between pump and heating tank, what is the dP minimum required for the control valve and the entrance to the heating tank when the heuristic mentions that at least 10 psi is required for the control valve? (c) The pressure at the inlet of the pump is 8 psi and the flowrate of the liquid produces pressure head of 50 psi. What is the total pressure produced by the pump? (d) Assume a pipeline dP of 2 psi / 100 ft for liquid flow in a pipe according to heuristic, what is the approximate maximum length of the pipe in ft that can be installed between the pump and the heating tank?

1 Answers  

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What is the best way to configure a bypass line in slurry services?

0 Answers  

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what is petrol density ? why ?

3 Answers   Reliance,

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CHEMICAL FLUID MECHANIC - EXAMPLE 3.1 : Water flows through a pipe with circular cross sectional area at the rate of V / t = 80 L / s where V is the volume and t is time. Let Av = 80 L / s where A is cross sectional area and v is velocity of fluid. For point 1, the radius of the pipe is 16 cm. For point 2, the radius of the pipe is 8 cm. Find (a) the velocity at point 1; (b) the velocity at point 2; (c) the pressure at point 2 by using Bernoulli's equation where P + Rgy + 0.5 RV = constant. P is the pressure, R = density of fluid, V = square of fluid's velocity, g = gravitational constant of 9.81 N / kg and y = 2 m = difference of height at 2 points. The pressure of point 1 is 180 kPa.

1 Answers  

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ACCOUNTING AND FINANCIAL ENGINEERING - EXAMPLE 34.18 : An investor is planning to purchase a small office for biochemical engineering consultancy on loan. In the calculation of the discount of payment in arrears, the following formula is used : y = 1 / (1 + r) ^ 1 + 1 / (1 + r) ^ 2 + 1 / (1 + r) ^ 3 + ... + 1 / (1 + r) ^ n where y = present value, r = interest rate of discount, n = number of payment, ^ = power used in certain computer languages for mathematics. (a) What is the meaning of : arrears? (b) Find a mathematical equation of y (1 + r). (c) Calculate, in less than 3 terms, y as a function of r and n.

1 Answers  

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