Explain various protein purification techniques?
Answers were Sorted based on User's Feedback
Answer / aroh shrivastava
Depending on the source, the protein has to be brought into
solution by breaking the tissue or cells containing it.
There are several methods to achieve this: Repeated
freezing and thawing, sonication, homogenization by high
pressure, clarification via cellulose-based depth filters,
or permeabilization by organic solvents. The method of
choice depends on how fragile the protein is and how sturdy
the cells are. After this extraction process soluble
proteins will be in the solvent, and can be separated from
cell membranes, DNA etc. by centrifugation. The extraction
process also extracts proteases, which will start digesting
the proteins in the solution. If the protein is sensitive
to proteolysis, it is usually desirable to proceed quickly,
and keep the extract cooled, to slow down proteolysis
| Is This Answer Correct ? | 14 Yes | 2 No |
What factors should be compared when evaluating cooling tower bids?
Question 82 - (a) The Hyperion sewage plant in Los Angeles burns 8 million cubic feet of natural gas per day to generate power in United States of America. If 1 metre = 3.28084 feet, then how many cubic metres of such gas is burnt per hour? (b) A reservoir of natural gas produces 50 mole % methane and 50 mole % ethane. At zero degree Celsius and one atmosphere, the density of methane gas is 0.716 g / L and the density of ethane gas is 1.3562 mg / (cubic cm). The molar mass of methane is 16.04 g / mol and molar mass of ethane is 30.07 g / mol. (i) Find the mass % of methane and ethane in the natural gas. (ii) Find the average density of the natural gas mixture in the reservoir at zero degree Celsius and one atmosphere, by assuming that the gases are ideal where final volume of the gas mixture is the sum of volume of the individual gases at constant temperature and pressure. (iii) Find the average density of the natural gas mixture in the reservoir at zero degree Celsius and one atmosphere, by assuming that the final mass of the gas mixture is the sum of mass of the individual gases. Assume the gases are ideal where mole % = volume % at constant pressure and temperature.
QUANTUM BIOLOGY - EXAMPLE 33.3 : In quantum biology, microtubule is used to store information in a cell. At temperature of T = 300 K, measured current I in a probe is directly proportional to the supplied voltage V, when passing through a microtubule with resistance R. (a) Form an equation of V as a function of I involving k as a constant. (b) If the microtubule has R = 1 ohm at such condition, find the value of V when I = 2 A. Hint : Ohm's law. (c) Find the relationship of k as a function of R.
Suppose you have hot oil and cold water, what kind fluid you flow In shell side and tube side and why?
What is a quick way to calculate frictional pressure drops in carbon steel pipe?
A concentric, counter-current heat exchanger is used to cool lubricating oil. Water is used as the coolant. The mass flow rate of oil into the heat exchanger is 0.1 kg / s = FO. For oil, the inlet temperature TIO = 100 degree Celsius and the outlet temperature TOO = 55 degree Celsius. For water, the inlet temperature TIW = 35 degree Celsius and the outlet temperature TOW = 42 degree Celsius. What is the mass flow rate of water in kg / s, FW needed to maintain these operating conditions? Constant for heat capacity of oil is CO = 2131 J /(kg K) and for water is CW = 4178 J /(kg K). Use the equation (FO)(CO)(TIO – TOO) = (FW)(CW)(TOW – TIW).
Chemical Engineering Energy Balance - Please match the term A - E as follow with the stated definition : A. Yield. B. Selectivity. C. Relative saturation. D. Molal saturation. E. Absolute saturation. i. Answer : ? (moles of desired product formed) / (moles that would have been formed if there were no side reactions and the limiting reactant has reacted completely). ii. Answer : ? (moles of desired product formed) / (moles of undesired product formed). iii. Answer : ? (relative humidity 40 % means partial pressure of water vapour equals 4 / 10 of the vapour pressure of water at the system temperature). iv. Answer : ? (moles of vapour) / (moles of vapour dry gas). v. Answer : ? (mass of vapour) / (mass of dry gas).
What are the customization steps in sap?
What are the main terms in unit operations? What are its characteristics?
What is the definition of 'good' cooling tower water?
What are some common problems associated with dense phase pneumatic conveying?
What are the apt definitions for apparent power, active power and reactive power? Explain about different types of lamps?
Civil Engineering (5086)
Mechanical Engineering (4453)
Electrical Engineering (16638)
Electronics Communications (3918)
Chemical Engineering (1095)
Aeronautical Engineering (239)
Bio Engineering (96)
Metallurgy (361)
Industrial Engineering (259)
Instrumentation (3014)
Automobile Engineering (332)
Mechatronics Engineering (97)
Marine Engineering (124)
Power Plant Engineering (172)
Textile Engineering (575)
Production Engineering (25)
Satellite Systems Engineering (106)
Engineering AllOther (1379)