Answer / Pradeep Kumar Bhati

Perfusion systems have several limitations that need to be considered: 1) Heterogeneity: Perfusion can lead to regional differences in oxygen and nutrient distribution, leading to heterogeneous cellular responses. 2) Shear stress: The flow of fluid through a perfused organ or tissue can cause mechanical stress on the cells, potentially damaging them. 3) Metabolic changes: The introduction of perfusate can alter the normal metabolic processes of the organ, leading to changes in cell function and viability. 4) Equipment complexity: Perfusion systems require specialized equipment that is expensive and requires technical expertise for maintenance and operation.

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