Answer / Mahesh Kumar Bhujel

ELISA (Enzyme-Linked Immunosorbent Assay) is an immunoassay used to detect and quantify specific antigens or antibodies in a sample. The mechanism involves coating a microplate with the antigen, adding the sample to bind to the captured antigen, and then adding an enzyme-labeled secondary antibody that recognizes the primary antibody in the sample. After incubation, a substrate is added, which is converted by the enzyme into a measurable product (color or luminescence), indicating the presence of the target antigen or antibody. ELISA can be used for various applications, including detecting infectious diseases, monitoring drug levels, and studying protein-protein interactions.

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