What are the main theoretical models that try to explain the formation of the enzyme-substrate complex?
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explain henderson-hasselbalch equation?
what is the difference between MUFA and PUFA?
In double bond, how many sigma and Pi bonds are present in it?
Which compound is involved in reducing levels of homocysteine in the blood?
what is the difference between reducing sugars and non reducing sugars?
Explain which type of chemical bond maintains the pairing of each chain in the dna molecule?
What happen to a denaturated enzyme regarding its functionality? How that result can be explained with the help of the lock and key model?
I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line
Splicing of pre-mRNA involves an unusual phosphodiester bond. Why is it unusual? What parts of the intron/exon form this bond?
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Which is element exhibit allotrope in the 6tha group?
Why does ion-exchange chromatography generally use a salt gradient or a pH gradient?
Explain the principle of ultracentrifuge?