Interested to Buy Any Domain ? << Click Here >> for more details...
explain henderson-hasselbalch equation?
- 1 Answers
- 7225 Views
- I also Faced
- E-Mail Answers
Answer / biomaster
The quantitative relationship among PH,buffering action of
amixture of weak acid and its conjugate base and the PKa of
the weak acid is given by a simple expression called
HENDERON-HASSELBALCH EQUATION.
The shape of titration curve of any weak acid is expressed
by Henderson-Hasselbalch equation.This is auseful way of
restating the expression for the dessociation constant of
an acid.
For the dessociation of aweak acid HA into H+ and A-,this
equation can be derived as follows.
Ka=[H+][A-]/[HA]
by rearraning the equation for solving H+
[H+]=Ka[HA]/[A-]
by converting to logarthmic functions
log [H+]=LOG Ka+log[HA]/[A-]
making expression negative
-log[H+]=-logKa-log[HA]/[A-]
SUBSTITUTING ph for -log[H=] and PKa for -logKa
PH=PKa-log[HA]/[A-]
NOW TO REMOVE MINUS SIGN INVERT LAST TERM I.E -LOG[HA]/[A-]
TO OBTAINHENDERSON-HASSELBALCH EQUATION.
PH=PKa-LOG[A-]/[HA];
| Is This Answer Correct ? | 5 Yes | 0 No |
explain why can it be said that the enzymatic action is highly specific?
Who were james watson, francis crik and maurice wilkins?
what is non competetive inhibition?
Some proteins profoundly alter the structure of DNA when they bind, bending it far out of its standard B configuration. Where do you think the energy comes from to distort the DNA?
1 Answers Pace Pharmaceuticals,
What is guaiac test and where it is used?
What are the favourable conditions for formation of anions?
What does law of mass action states?
Explain RS notation of isomers?
What are the differences and respective advantages to electrophoresis, SDS PAGE, and isoelectric focusing?
What is the tertiary structure of a protein?
Compare endonuclease and exonuclease?
what are the disadvantages of immobilized enzymes
Micro Biology 
