Interested to Buy Any Domain ? << Click Here >> for more details...
Explain the principle of ultracentrifuge?
- 1 Answers
- 26653 Views
- I also Faced
- E-Mail Answers
Answer / biomaster
The ultracentrifuge method for determining the molecular
weights of proteins was developed by svedberg.
in this method protein molecules are subjected to
gravitational forces greater in magnitude than thermal
forces whereby causing them to diffuses.protein molecules
are larger in size called macromolecules.
under high centrifugal forces these molecules can be made
to sediment towards one end of a centrifuge tube.
The equation derived by svedberg for the molecular weight
of a protein is
M=RTS/D(1-Vp)
where R=gas constant
T=absolute temperature
s=sedimentation constant
D=diffusion constant
V=partial specific volume ofprotein
p=density of solvent
PRINCIPLE OF ULTRACENTRIFUGE IS "For most proteins however
the value of V is in the range of 0.70 to 0.75".
| Is This Answer Correct ? | 53 Yes | 16 No |
What are the structural and stability consequences of electron delocalization in amides (peptide bonds)?
what is siderophilin?
How does the formation of the enzyme-substrate complex? Explain the reduction of the activation energy of chemical reactions?
What features do the helix and the sheet have in common?
how will u identify which one of the given 2 test tube contains protein solution or which one contains DNA solution without using any instrument?
What is the anhydride of n2o5?
What is allotropy?
Which is the most Abundant Alkaline earth element?
you have a mixture of dNTPs(deoxynucleotide triphosphates)in a sample.how can you separate these all?
What factors contribute to the entropy change when a denatured protein folds up?
Explain what does thermodynamics helps in predicting?
According to the fluid mosaic model of cell membranes, which type of molecule spans the membrane from its inner to outer surface? a) cholesterol b) phospholipid c) protein d) triacylglycerol
Micro Biology 
