I have given the protocol for the cyclodextrin glygosyl
transferase assay:
One ml of appropriately diluted enzyme sample was incubated
at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized
soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction
was terminated by boiling the reaction mixture for 3 min
and reaction volume was made to 10 ml with distilled water.
Two ml of above reaction mixture was withdrawn and mixed
with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and
0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml
absolute alcohol). Absorbance was measured at 550 nm. The
percent decrease of sample was calculated with respect to
control containing 5 ml of buffer, 5 ml of sodium carbonate
and 0.5 ml of phenolphthalein.
where Acontrol = absorbance of control and Atest =
absorbance of sample.
The amount of β-cyclodextrin (β-CD) produced was estimated
from the standard graph of 0–500 μg/ml β-CD concentration
against % decrease in absorbance. One unit of CGTase was
defined as the amount of enzyme required to produce 1 μm of
β-CD/min.
Please can you suggest me the formula for the defination
given in the last line
No Answer is Posted For this Question
Be the First to Post Answer
how shikimic acid is synthesised?
How does the stability of peptides compare with that of amino acids?
Explain the Use of ammonal?
CHEMICAL STRUCTURE OF TREHALOSE?
What are nucleic acids? What is the historic origin of this name?
Explain kararch effect?
What are some common modifications made to sugars in GAGs?
what is the chemistry of cellulose?
1 Answers Biological.e.limited,
what are lac operons?
5 Answers Biological.e.limited,
what is Signal Recognition Particle?
Define bond energy?
southern blot technique is related to--------- [fill the gaps]