What are the favorable conditions for formation of Anions?

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What factors contribute to the entropy change when a denatured protein folds up?

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Explain who proposed law of mass action? What does it states?

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Which is the most stable sulphur at room temperature?

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if hydrogen ion concentration of a solution is 90% what will be the ph of that solution?

2 Answers   BITS,

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What types of things happen to proteins during posttranslational processing (hydroxylation, etc.)?

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what are the reasons for gaucher's disease?

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What is the difference between myoglobin and haemoglobin in the sequence of poly peptides?

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How the pure culture can be produced?

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southern blot technique is related to--------- [fill the gaps]

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Which cellular compartment becomes acidic during mitochondrial electron transport?

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I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line

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