How does temperature affect the action of enzymes upon their substrates?

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I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line

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what are the different types of calines?

1 Answers   Sun Pharma,

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What can you calculate by selecting Born-Haber cycle?

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which is antiegg injury factor?

1 Answers   Manoj Pharma Ltd,

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suppose you are given a bucket of water having volume 10 litre.In that bucket aprotein sample of unknown concentration was mixed.how will you determine the concentration of protein from that bucket??

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what is racemic mixture and what is racemisation?

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What is a plasmalogen? What do plasmalogens do?

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What are pentoses? To what organic group do pentoses belong? Are nucleotides formed of only one type of pentose?

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Which branch of science deals with the transformation of chemical energy into electrical energy and vice versa?

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what is the chemical formula of kinetin?

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is there a hormone in our body which when released induces sleep in us?is there a spurt in the secretion of that hormone in adolescence?why?

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