How much carbon loading in nomalphase columns?
- 5 Answers
- 5814 Views
- I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / ajinkya
it contain only silica, and in reverse phase c8 and c18
| Is This Answer Correct ? | 1 Yes | 1 No |
Answer / umesh chand
Normal phase column have no number of carbon ,its having silica gel particle coated with cellulose (3,5-di methylephenylcarbamate) and amylose (3,5-di methylephenylcarbamate)
like CHIRALPAK-OJ-H,CHIRALPAK -IA ,IB ,IC and etc.
'' polar stationary phase and nonpolar mobile phase'' use in normal phase chromatography
but carbon having hydrophobic nature, therefor we can't carbon loading in normalphase column.
| Is This Answer Correct ? | 1 Yes | 1 No |
why multimedia dissolution require to do?
why results are variation in same samples analysis in gc and hplc ?
For LOD and LOQ determination which method is accurate? (slopemethod or s/n ratio method)
In my analysis by HPLC,In sample main peak and placebo peak are eluting with shorter resolution ( almost look like doublet). how can i get max resolution ? Is changing gradient method is usefull?
From where 16 or 5.545 is derived in theoretical plates calculation formula by USP or JP/BP/DAB and what is meaning of this coefficient and impact in calculation ?
2 Answers Nectar Lifesciences,
2. Two grams of Benzoic acid are dissolved in 200 ml of water and extracted with 200 ml of diethyl ether. The distribution coefficient of benzoic acid is 100, and its dissociation constant is 6.5 10-5. Calculate the distribution ratio (D) of benzoic acid at pH 2, 5, and 6. 3. Calculate D at pH 2 to 10 (1 unit apart) in the above problem, and plot D versus pH.
Why we are having specification 100% above in case of assay as there is only 100% of compound?
2 Answers MSN Pharma, Nectar Lifesciences,
What is stray light in uv what are units for stray light
about method developement in hplc
control of resolution in calibration of IR
Nitrogen test is used for which chromatography?
How to check hplc detecter sensitivity?
Organic Chemistry 
