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Why methanol & acetonitrile is mostly used for hplc analysis?
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Answer / ankur dwivedi
Most substances are easily soluble in these solvents. They have lower absorbance in routine HPLC working range (205-400 nm). Hence no interference will be there due to these solvents.
Due to less density, they create less pressure in column and hence the column life can be improved.
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Drug substance/product easily dissolve in it.
Lower absorbance.
these solvents have lower wavelengths,whish compounds below 210nm these may helps to elute first from the column.As a results it will not effect pressure on the column
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