Why Urasil and Caffeine use for HPLC wavelength accuracy
calibration. which is most suitable, Why? other than these
which can be used?

Answers were Sorted based on User's Feedback



Why Urasil and Caffeine use for HPLC wavelength accuracy calibration. which is most suitable, Why? ..

Answer / hemadri

Because it is stable in any condition . Easily availability in market. It will give sharp peak and run time also less.

Is This Answer Correct ?    13 Yes 3 No

Why Urasil and Caffeine use for HPLC wavelength accuracy calibration. which is most suitable, Why? ..

Answer / suresh polisetti

bcoz these r gives the both abs max & min in the UV Region which means max= 205,273,min=245,

Is This Answer Correct ?    10 Yes 0 No

Why Urasil and Caffeine use for HPLC wavelength accuracy calibration. which is most suitable, Why? ..

Answer / manish

caffeine and urasil solution is more stable at rt and show
multiweve length .

Is This Answer Correct ?    4 Yes 1 No

Why Urasil and Caffeine use for HPLC wavelength accuracy calibration. which is most suitable, Why? ..

Answer / rishabh

anthracene and holomium oxide can also used for wavelength accuracy detector

Is This Answer Correct ?    0 Yes 0 No

Why Urasil and Caffeine use for HPLC wavelength accuracy calibration. which is most suitable, Why? ..

Answer / mahendra

Because it give dual wavelength absorbance like 245nm 350 nm

Is This Answer Correct ?    5 Yes 12 No

Why Urasil and Caffeine use for HPLC wavelength accuracy calibration. which is most suitable, Why? ..

Answer / chemistry

Because uracil And Caffierne Shows The absorbance Range in
245nm And 340nm.

Is This Answer Correct ?    0 Yes 7 No

Why Urasil and Caffeine use for HPLC wavelength accuracy calibration. which is most suitable, Why? ..

Answer / gaurav oza

caffeine and urasil does not retain in HPLC. They are
generally used for measuring the dead volume or void volume
, so both are used for calibration of HPLC column

Is This Answer Correct ?    2 Yes 10 No

Post New Answer

More Analytical Chemistry Interview Questions

how will you do the prep for unstable componds?

0 Answers   AMRI, CTX Life Sciences, Piramal Healthcare,


1.What is the difference between method validation and method verification 2.Which guidelines proposed to method transfer

0 Answers   Granules,


Why sodium lamp is used as source of Polarimeter?

12 Answers   College School Exams Tests, Hetero, NCL, Zydus Cadila,


if peak get problamatic then what require to do?

0 Answers  


iam using ph instrument lab india make.3ponit and 5point.in calibration time 3point slope will come 98.89and 96.87.that limit is 85 to 105.what basis slope will come.any calucalation between them.5point wiicome slope 99.89?why 3p0int came 2 values

1 Answers  






What is peak purity and response factor in chromatographic analysis? Why we required to find out response factor?

2 Answers  


How to calibration of the uv spectroscopy and its test?

0 Answers  


what is difference between polarity and dipolemoment

3 Answers   Neuland,


How do you show that equal quanitities of two isomers provide the same response using a PDA

1 Answers  


What are usp limits for total impurities,unknown impurity

1 Answers   Dr Reddys,


How to calculate %w/w and %v/v of a component(or solvent)by NMR

1 Answers   ACME, Vimta Labs,


Why void volume(time in seconds) 1 in hplc system suitability

1 Answers  


Categories
  • Organic Chemistry Interview Questions Organic Chemistry (342)
  • Inorganic Chemistry Interview Questions Inorganic Chemistry (123)
  • Analytical Chemistry Interview Questions Analytical Chemistry (1387)
  • Physical Chemistry Interview Questions Physical Chemistry (62)
  • General Chemistry Interview Questions General Chemistry (478)
  • Chemistry AllOther Interview Questions Chemistry AllOther (190)