Why Urasil and Caffeine use for HPLC wavelength accuracy
calibration. which is most suitable, Why? other than these
which can be used?
Answer Posted / rishabh
anthracene and holomium oxide can also used for wavelength accuracy detector
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2. Two grams of Benzoic acid are dissolved in 200 ml of water and extracted with 200 ml of diethyl ether. The distribution coefficient of benzoic acid is 100, and its dissociation constant is 6.5 10-5. Calculate the distribution ratio (D) of benzoic acid at pH 2, 5, and 6. 3. Calculate D at pH 2 to 10 (1 unit apart) in the above problem, and plot D versus pH.
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