How will you select mobile phase in HPLC Method Development

Question Posted / ramesh

4 Answers
18077 Views
I also Faced
E-Mail Answers

Answers were Sorted based on User's Feedback

How will you select mobile phase in HPLC Method Development..

Answer / maulik

based on the pka value of compound. if ur compound have pka
is about 3. u can select ur mobile phase about ph 5-14.but
due to the less column selectivity in basic media u can
select 5-8 pH. preferred by the different salt buffering
capacity.

Is This Answer Correct ?

19 Yes

5 No

How will you select mobile phase in HPLC Method Development..

Answer / kkpk

Based on the solubility of the drug.

Is This Answer Correct ?

20 Yes

11 No

How will you select mobile phase in HPLC Method Development..

Answer / gajesh patel

Dependent upon pKa value.

Is This Answer Correct ?

10 Yes

1 No

How will you select mobile phase in HPLC Method Development..

Answer / rishabh

First of all check your compound solubility in which solvents then after that based on solubility the Mphase selection becomes easy if compound is soluble in aqueous medium check the pH of that medium than after further procced if not then check the solubility in organic solvents on basis of solubility and pH of the Mobile phase. Both Mobile phase selection and stationary phase are selected.

Is This Answer Correct ?

0 Yes

0 No

Post New Answer

More Analytical Chemistry Interview Questions

some compound havving uv region but not give much more response during hplc analysis

2 Answers

In the HPLC Calibration done wavelength accuracy done between 200nm-280nm .but not done remaining 300-400nm not done ?

0 Answers

In Analytical method development related substance method when did follow w/w method when did follow area normalisation method

2 Answers Dr Reddys, Sun Pharma,

if tech transfer fails whay require to do?

0 Answers

Retention time & Relative Retention time Definition & between difference? How to Calculate response factor(RF) & relative response factor(RRF)?

3 Answers MSN Pharma, Titan,

what is the difference between LOSS ON DRYING IN ORDINARY OVEN TECHNIQUE AND VACUUM OVEN TECHNIQUE

6 Answers

What is NPOC method in TOC?

1 Answers Zydus Cadila,

what is the general mechanism of high performance liqiud chromatography?

7 Answers Ajanta Pharma, Amneal Pharmaceuticals,

How to determine retention time in Chromatography

2 Answers Mylan,

What is pooled sample in Dissolution

2 Answers Graviti Pharmaceuticals, Lupin,

what is the disso medium for tropsiun chloride

0 Answers

What is the Defination of Tapdensity ?

4 Answers Dr Reddys, R L Fine Chem,

For more Analytical Chemistry Interview Questions Click Here