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why the same column give different retention time in hplc?
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Answer / nabil
there are many resons for different RT
1-The pressure used(because that affects the flow rate of
the solvent)
2- The nature of the stationary phase(not only what
material is made of, but also particle size.
3-the exact compositionsof the solvent.
4-the temp of the column.
| Is This Answer Correct ? | 23 Yes | 0 No |
Answer / prabhakar
Because preparation of mobile phase variation(mostly)
| Is This Answer Correct ? | 6 Yes | 4 No |
Answer / dhaval bhatt
1)column temp
2)presser not stable
3)leakage column from any side
| Is This Answer Correct ? | 1 Yes | 0 No |
Answer / vaibhavgoswami
due to pressure ,
different column packing,(silica)
different maker
| Is This Answer Correct ? | 0 Yes | 0 No |
if mobile not sonicated properly the retention time will vary
| Is This Answer Correct ? | 1 Yes | 3 No |
Answer / phanindra
I think it is due to contamination of HPLC column remedy is column kept for wash with 100%polar solvent.
| Is This Answer Correct ? | 0 Yes | 6 No |
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