Follow Our FB Page << CircleMedia.in >> for Daily Laughter. We Post Funny, Viral, Comedy Videos, Memes, Vines...


why the same column give different retention time in hplc?

Answers were Sorted based on User's Feedback



why the same column give different retention time in hplc?..

Answer / nabil

there are many resons for different RT
1-The pressure used(because that affects the flow rate of
the solvent)
2- The nature of the stationary phase(not only what
material is made of, but also particle size.
3-the exact compositionsof the solvent.
4-the temp of the column.

Is This Answer Correct ?    23 Yes 0 No

why the same column give different retention time in hplc?..

Answer / srinivas

many reaons for different RT in same column
1) Depend on PH in buffer Preparation
2) copmposition of buffer and solvent
3) difference in manual injector and auto injector

Is This Answer Correct ?    3 Yes 0 No

why the same column give different retention time in hplc?..

Answer / prabhakar


Because preparation of mobile phase variation(mostly)

Is This Answer Correct ?    6 Yes 4 No

why the same column give different retention time in hplc?..

Answer / dhaval bhatt

1)column temp
2)presser not stable
3)leakage column from any side

Is This Answer Correct ?    1 Yes 0 No

why the same column give different retention time in hplc?..

Answer / vaibhavgoswami

due to pressure ,
different column packing,(silica)
different maker

Is This Answer Correct ?    0 Yes 0 No

why the same column give different retention time in hplc?..

Answer / ravi kumar tummala

if mobile not sonicated properly the retention time will vary

Is This Answer Correct ?    1 Yes 3 No

why the same column give different retention time in hplc?..

Answer / phanindra

I think it is due to contamination of HPLC column remedy is column kept for wash with 100%polar solvent.

Is This Answer Correct ?    0 Yes 6 No

Post New Answer

More Analytical Chemistry Interview Questions

How to calculate LOD & LOQ in HPLC Validation Method

14 Answers   Dr Reddys, GSK GlaxoSmithKline, Reddy Labs, Reliance, UIS,


from compund pka value how to select buffer and column?

2 Answers   Emcure, Zydus Cadila,


How can you calculate manually "Slope" in pH calibration?

2 Answers   Hetero,


what is the difference between c-8 and c-18 column

7 Answers  


How did you conclude that when stressing a compond, all degradation products have eluted in chromatogram?

7 Answers   Matrix,






in monograph 7 imp are there but in vendor showing 5 imp do not req to include 2 imp for that what will be justification?

0 Answers  


In which situation we require to prepare the standard solution from sample in Related substance method?

0 Answers  


What is the deference between Residual solvents and OVI

1 Answers   RA Chem Pharma,


Why we are using the methanol in Kf titration?

6 Answers   Reddy Labs,


Why do we use KMnO4 in the test of control of obsorbance ? and why do we take specific quantity i.e 57-63mg?

0 Answers   Reddy Labs, Vimta Labs,


control of resolution in calibration of IR

3 Answers  


k.f principle in finding out moisture content

1 Answers  


Categories
  • Organic Chemistry Interview Questions Organic Chemistry (302)
  • Inorganic Chemistry Interview Questions Inorganic Chemistry (123)
  • Analytical Chemistry Interview Questions Analytical Chemistry (1385)
  • Physical Chemistry Interview Questions Physical Chemistry (62)
  • General Chemistry Interview Questions General Chemistry (478)
  • Chemistry AllOther Interview Questions Chemistry AllOther (190)