Answer / satheesh kumar

Post run in hplc comes under the gradient programme
(Gradient elution).

Gradient Programme-:

Time(min) A% B%(Where-A% Buffer,B%-ACN)

0.01 80 20
10.00 50 50
25.00 20 80
30.00 20 80
35.00 80 20
45.00 80 20

In the above gradient Intially starts with Buffer,ACN in
one proportion,when the time incerases proportion changed
and finallty returns to original proportion is called Post
run or equilibrqtion time

what is leading peak in hplc

1 Answers   Cipla,

---

is a bonded water molecule in an organic material can be easlisy and accuractly determined by KF rather than LOD

2 Answers  

---

Why do we need GC instead of HPLC? or Why do we need HPLC instead of GC?

4 Answers  

---

why using Potentometry What Purpose? differance between manual and potentiometry? why using Some of materials in usig potentiometry? What is use of mericuric acetate in Potentiometry

1 Answers   Biocon,

---

why sucrose used in polarimeter calibration?

2 Answers   Reddy Labs, Sugar Factory,

---

What is the reason for negative peak in HPLC while using UV detector.

2 Answers  

---

Difference between uv detector nd RI DETECTOR? wt s the difference between OVI Solvents nd residual solvents incase of GAS CHROMATOGRAPHY? nd wt s its detection limit?

3 Answers  

---

what is mean by profiling of dissolution

1 Answers  

---

what are the standard methods for HPLC?

0 Answers   Orchid,

---

what are peak purity,peak purity index,peak thresold

2 Answers  

---

What is the difference between uplc and hplc

4 Answers   AAU Assam Agricultural University, Hetero,

---

What is FFA, PV, and PAV value in oil.

1 Answers   Nestle,

---