Follow Our FB Page << CircleMedia.in >> for Daily Laughter. We Post Funny, Viral, Comedy Videos, Memes, Vines...


WHEN AN UN KNOWN SAMPLE IS GIVEN TO U,HOW WILL U START THE
ANALYSIS(ie FIRST WHICH TEST TO BE DONE,NEXT,THEN NEXT),HOW
WILL U DECIDE WHICH TYPE OF ANALYSIS TO BE DONE FOR IT?

Answers were Sorted based on User's Feedback



WHEN AN UN KNOWN SAMPLE IS GIVEN TO U,HOW WILL U START THE ANALYSIS(ie FIRST WHICH TEST TO BE DONE..

Answer / gaurav oza

step-1 Organoleptic test- color, odour, shape.
step-2 test of functional groups.
step-3 confermatory test for functional groups.
step-4 qualitative tests for presence of compounds.
step-5 IR spectroscopy
step-6 UV spectroscopy and titration methods for
quantitative tests.
step-7 HPLC for better results and impurity study.

Is This Answer Correct ?    19 Yes 1 No

WHEN AN UN KNOWN SAMPLE IS GIVEN TO U,HOW WILL U START THE ANALYSIS(ie FIRST WHICH TEST TO BE DONE..

Answer / dhananjay s chavan

Pre method development requirements
1 Literature search
2 Published method
3 Availability of Pharmacopiel/Supplier/DMF Impurities

4 Availability of Pharmacopiel/Supplier/DMF Method

5 Workability of Pharmacopiel/Supplier/DMF Method
6 Identify different source of API
7 API degradation data from Supplier/DMF
8 API stability data from Supplier/DMF
9 Calculate imp level as per ICH for FPS

Method development
10 API Solubility in different pH
11 API Solubility in different solvent
12 Placebo interference
13 Main peak recovery/peak shape/response
14 Degradation study in different condition with
autoclave
Achieve degradation between 5 to 20%
Peak purity of all known impuriite(s) and main peak

Peak purity of all unknown impurities above LOQ
level
Check the elution of all degradants by extending
runtime
15 Establish LOD/LOQ
16 Filter study
17 Elution of all source impurities in final method

18 Reproducibility of method in different system and
different column lot
19 Check System suitability requirement (Resolution,
Similarity factor, Std RSD…etc)

Parmeteres to be done on finlized method
20 RRF Development in different system
21 LOQ, 100% and 150% Recovery in duplicate
22 Linearity
23 Stability sample and different batch analysis
24 Solution stability
25 Robustness in final method (Flow/temp./pH variation)

26 Method equivalency between final method and other
method(s)
27 Method evaluation by validation team

Is This Answer Correct ?    3 Yes 9 No

Post New Answer

More Analytical Chemistry Interview Questions

formula for %RSD.

4 Answers   Laurus,


stability study is going on up to 3years for famotidine-USP but the pharmacopeia is revised and tlc test is replaced with hplc test. At the 4th year frequency the product is failed in hplc test .how can i assain the expiry or retest date and can i stop or continue the programme?

2 Answers   Nakoda Chemicals,


Describe your field experience sample type collected,sample techniques,field measurements taken and equipment used?

0 Answers   Apex, BNM, Ranbaxy,


In Agilent HPLC calibration using Empower 3 Software, What is the criteria for GPV Baseline starting point? i.e., Either it can start from -0.00/+0.00 or any range is there like -0.05 to +0.05 or ......etc?

1 Answers   Apotex,


what is mean by extactable and leachable study?

0 Answers  






How they found 1mL of K.F reagent is equivalent to 5mg of water and if we change the composition of K.F reagent, is it can neutralize more amount of water?

0 Answers  


what r the reasons to get peak telling?

6 Answers   Cipla,


How to choose Normal phase or Reverse Phase HPLC method Conditions for given sample?

3 Answers   PharmaZell,


What is difference between chemical Assay and Hplc assay?

2 Answers   Lee Pharma,


How to calculate the signal to noise ratio.

1 Answers   Rakshit Drugs,


why RID detector is used for selective materials like Mannitol, Hydroxyethyl Starch and Sorbitol?

5 Answers  


What r the limitatoins of uv visible spectroscopy ? Why Water use in blank standard for uv visible spectroscopy ?

4 Answers   Cipla, Reddy Labs,


Categories
  • Organic Chemistry Interview Questions Organic Chemistry (302)
  • Inorganic Chemistry Interview Questions Inorganic Chemistry (123)
  • Analytical Chemistry Interview Questions Analytical Chemistry (1385)
  • Physical Chemistry Interview Questions Physical Chemistry (62)
  • General Chemistry Interview Questions General Chemistry (478)
  • Chemistry AllOther Interview Questions Chemistry AllOther (190)