Answer Posted / satheesh kumar
Post run in hplc comes under the gradient programme
(Gradient elution).
Gradient Programme-:
Time(min) A% B%(Where-A% Buffer,B%-ACN)
0.01 80 20
10.00 50 50
25.00 20 80
30.00 20 80
35.00 80 20
45.00 80 20
In the above gradient Intially starts with Buffer,ACN in
one proportion,when the time incerases proportion changed
and finallty returns to original proportion is called Post
run or equilibrqtion time
| Is This Answer Correct ? | 13 Yes | 2 No |
Post New Answer View All Answers
is it nessesary to do solution stability for 7 days?
in stress study if your api not soluble in hcl naoh h2o2 then what require to do?
what is the acceptance criteria for enteric coated tablets in 0.1n hcl validation in each parameter?
How to set analyticl specification for combination products?
What is the diffrence in japan mkt requirement in analytical method validation over US?
Why Ethanol is Used for Standardization of GC Head space?
how to calibrate hplc & gc
What is mean by PDR?
WHY given much noise PDA dector then VWD Dector
How we choose the mobile phase for method development.?
If change in specification which parameter require to do for validation? if change in chromtographic condition then which parameter? if api change then which parameter? if change composition then which parameter? if old method not work out then whicj parameter? if additional one impurity added then which parameter of validation require to do on above each conditions? elaborate separately
How will u perform degradation study in detectors other thann PDA in HPLC Methodology
what is the different in Total ash, sulphated ash, acid insoluble ash, alcoholic or non alcoholic ash?
what are the standard methods for HPLC?
which one is better separation in high pressure liquide chromatogram orlowpressure chromato gram in hplc