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What r the limitatoins of uv visible spectroscopy ? Why
Water use in blank standard for uv visible spectroscopy ?
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Answer Posted / tanu bhardwaj
when we prepare a solution for UV-vis spectroscopy, we prepare it in a solvent, to remove the absorption effects due to solvent, we keep that solvent into blank, so that when the two rays coming out of two cuvettes are subtracted, then we get only the absorption due to solute, not due to solvent. if we prepare sample in water, then water is used as blank.
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