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Why the same type of columns showing different RT for a single
product?
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Answer Posted / amol
because , when manufacturing of column is done different
parameter is affected which is given below , due to this
each column is unique tho it is same type.
column parameter
column pressure
parical size
carbon density
dead voulume of the column
end fitting
end capping of the column.
silica used.
Hplc System Parameter
length of tubing.
internal dia. of tube.
void volume of system.
pump working.
flow rate.
end fittings.
ETC....
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