Why the same type of columns showing different RT for a single
product?
- 6 Answers
- 12254 Views
- I also Faced
- E-Mail Answers
Answer Posted / reet
following factors might be the reason:
1. Column ageing
2. how the column was preserved, or what mobile phase was
used before the present experiment
3. sample preparation might be the cause. sample may be non-
homogeneous
4. Slight variation in thepH may also affect the variation
in RT.
5. Other parameters like,temperature can also be one of the
reason.
6. last but an imporatant factor might be some air bubble
trapped in the system.
| Is This Answer Correct ? | 3 Yes | 2 No |
Post New Answer View All Answers
in sampling of raw material which side is pasted sampled labels
How to set analyticl specification for combination products?
In IR spectra, generally a molecule vibrates without apply emr or ir light?
how to develop the icp ms method? Application of icp ms?
In HPLC (Waters) software Empower1,2 &3 what is the updation for each version
inhouse product is in capsule form in combination and RLD is in tablet form then can we proceed for multimedia CDP? in inhouse capsule product disso is paddle with sinker in release media is there then RLD product in tablet form then with same as paddle with sinker we can proceed n.a.?
What is related substance by HPLC impurity limits as per USP?
all types of questions asked at the cipla interview
please explian me about area normalisation method,diluted standard method,impurity vs impurity and which one has to be selected in method development
If change in specification which parameter require to do for validation? if change in chromtographic condition then which parameter? if api change then which parameter? if change composition then which parameter? if old method not work out then whicj parameter? if additional one impurity added then which parameter of validation require to do on above each conditions? elaborate separately
what is biorelivent dissolution media?
What is aggregate and fragments in SEC?
If vendor having more imp than monograph then how to proceed? and how to set spec?
calibrtion procedue of LC-MS
how to qualify the impurity?
