What is the principle of Autoclave
Answers were Sorted based on User's Feedback
Answer / indu madhavi
main principle of autoclave is steam under pressure &the
tem is 121degrees centigrade 15lbs pressure for 15 min will
kill the micro organisms
| Is This Answer Correct ? | 322 Yes | 35 No |
Answer / maitri
Autaclaving is done for sterilization of culture media and
the glass equipments.The main principle is to maintain high
temperature and high pressure inside an equipment. The
maintained pressure is 15lbs and temperature is 121^C. In
that condition of temperature and pressure the
microorganisms are killed.
| Is This Answer Correct ? | 160 Yes | 23 No |
Answer / avinash
The main principle involved in autoclaving is,sterilization
i.e, inorder to kill the microorganisms.The temperature
level is 121C and the pressure level is 15lbps,after
reaching 15lbps it should be kept for still 15min,then it
will be completely sterilized!!!!!!
| Is This Answer Correct ? | 96 Yes | 17 No |
Answer / sakisaleem
Autoclave is a device used for strilization of equipment
and media under the high pressure steam 121 °C or more, for
15 to 20 minutes depending on the size of the load and the
contents.
| Is This Answer Correct ? | 67 Yes | 8 No |
Answer / patel aditi
autoclave is used for sterilization of media and glass wares
which are used in laboratory. it work at 121degree
centigrate temprature and 15 lbs pressure. at this condition
all the micro organism and their spores are killed.
| Is This Answer Correct ? | 42 Yes | 11 No |
Answer / shriniwas
the main principle of autoclave is to sterilize the
material under a very high steam pressure so that it can
easily penetrate the material and destroy the micro
organism.the temperature of 121 degree celcius/15 ibs
pressure is maintained for 15 min for the complete
destruction of the micro organism.
| Is This Answer Correct ? | 28 Yes | 3 No |
Answer / perry
main principle of autoclave is mosit sterlization steam
under pressure & temperature is 121degrees centigrade 15
Lbs pressure.AT THIS TEMPERATURE IT WILL destroy the
thermophillic bacterias also if we exceed the temperature or
pressure it will degrade our media
| Is This Answer Correct ? | 26 Yes | 2 No |
Answer / sk. wazed ali mollah
sterilization under 121ºc and 15lb pressure for 15
minute,which killed all viable cells and spores.
| Is This Answer Correct ? | 39 Yes | 16 No |
Answer / ravindrasing
Moist heat steriization under specific (15psi) pressure at
121.1Degrees for 20 minutes.
| Is This Answer Correct ? | 22 Yes | 8 No |
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