Bio Chemistry Interview Questions
Questions Answers Views Company eMail

what is a chiral molecule?

Reddy Labs,

7 5886

what is lineweaver burk equation?explain it?

1 4513

what is meant by palindromic dna?

2 2961




how do u differentiate pseudoglobulins and euglobulins on the basis of solubility?

1 2651

what are muco polysaccharides?

2 5610

who is considered as the father of modern biochemistry?

8 15272

what is acidosis and alkalosis in terms of PH?

2 2892

what is the PH of bile?

Biological.e.limited,

2 4380




explain henderson-hasselbalch equation?

1 4966

what is nucleophilic attack?

2 2669

what are eicosanoids?name the three subclasses of eicosanoids?

Cipla,

3 9575

explain about hyperglycemic agent?

1 1852

what is the procedure of aschheim-zondec test?

1 3321

what is meant by panhypopituitarism?

1 1976

what are different types of goitrogens?

Biocon,

2 4790


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Un-Answered Questions { Bio Chemistry }

Why is the t-butyloxycarbonyl protecting group such a good choice for amino acid synthesis?

963


why we use 96 well plate in ELISA plate reader?

1104


What are hydrophobicity scales, and how are they used?

749


why life span of RBC IS GREATER THEN THE WBC?

1392


what is your aspiration in profession/ career?

1518






What type of column is generally used to separate amino acids from each other?

960


What are species grow in waitland?

1218


why we use 96 well plate in ELISA plate reader?

1118


Two children have a neurological disorder. When cells from the two patients were fused to form heterokaryons containing nuclei from both patients and cultured, the cells displayed normal metabolism. Cells from either patient exhibited abnormal metabolism when cultured separately.what does these results indicate?

943


How are free energy, equilibrium and spontaneity related to each other?

962


I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line

914


The nucleophile in the first stage of the serine protease mechanism is a) H2O b) the carboxyl of Asp 102 c) the hydroxyl of Ser 195 d) the imidazole of His 57

947


The elution volume of an enzyme on a gel filtration column can be predicted from a) its enzyme activity b) its protein absorbance at 280 nm c) subunit composition and monomer molecular mass(es) d) Choices a) and b) are both correct.

1909


Describe the structure of a peptide giving the sequence.

714


Glycolysis is A) C6H12O6 --> 2C2H5OH + 2CO2 B) C6H12O6 --> 2CH3H4O3 + 4H c) C3H4O3 + NAD --> C2H5OH + CO2 NAD D) C6H12O6 + 6H20 --> 6CO2 + 6H2O

1431