what is the difference in the aminoacid sequence in normal haemoglobin and sickle cell anaemic person?
3 3502Post New Bio Chemistry Questions
why we use 96 well plate in ELISA plate reader?
Why are right-handed helices more stable than left-handed helices?
why life span of RBC IS GREATER THEN THE WBC?
What factors are involved in determining the electrophoretic mobility of a molecule?
How do hydrophobic and hydrophilic residues arrange themselves in globular proteins?
How are free energy changes related to LeChatelier's Principle?
How is the actual free energy change for a process related to the standard free energy change?
Glycolysis is A) C6H12O6 --> 2C2H5OH + 2CO2 B) C6H12O6 --> 2CH3H4O3 + 4H c) C3H4O3 + NAD --> C2H5OH + CO2 NAD D) C6H12O6 + 6H20 --> 6CO2 + 6H2O
I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line
What do you like LEAST & MOST about your current position?
How does hplc differ from normal column chromatography, and what are its advantages?
In which forms arsenic impurities present in drug.
Which organ and subcellular site are most important for fatty acid biosynthesis?
How are coupled reactions used in biochemistry?
Why are free cysteines treated with iodoacetate prior to protein sequencing?
