what is the difference in the aminoacid sequence in normal haemoglobin and sickle cell anaemic person?
3 3316Post New Bio Chemistry Questions
I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line
what is meant by codan optimisation m rna enrichment?
Why is the t-butyloxycarbonyl protecting group such a good choice for amino acid synthesis?
Which organ and subcellular site are most important for fatty acid biosynthesis?
I understand that urine can act as a reagent to break disulfide bonds? I am particularly interested in bonds between cysteines. and or any other amino acids.
can i used antimony potassium tartrate against potassium tartrate in Fehling B solution ?
All technical,written test questions
How is the actual free energy change for a process related to the standard free energy change?
Why are right-handed helices more stable than left-handed helices?
what is QC and why is impotent in pathology lab
Calculate the molar extinction coefficient of a solution containing 5 *10-4 g litre-1 of a biomolecule, molecular weight 275 g mol-1, and absorbance 0.75 in a 1.2 cm cuvette.
Describe the structure of a peptide giving the sequence.
How does a random coil differ from an irregularly structured region?
What type of column is generally used to separate amino acids from each other?
What are species grow in waitland?
