What are enzymes? What is the importance of enzymes for the living beings?
Which branch of science deals with the transformation of chemical energy into electrical energy and vice versa?
What does ammonal contain? For what purpose it is used?
Explain what are similarities and differences between the transcription process and the replication processes?
What are the main factors that alter the speed of enzymatic reactions?
Explain which type of chemical bond maintains the pairing of each chain in the dna molecule?
What is meant by the 5’ and 3’ extremities of nucleic acids?
What does thermodynamics helps in predicting?
Which enzymes in cows allow them to digest cellulose?
What is the lattice energy of nacl?
Define kararch effect?
What are respectively some remarkable functions of keratin?
I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line
What is the chemical formula of xylose?
What is banana bond?
