Bio Chemistry Interview Questions
Questions Answers Views Company eMail

wat r the applications of isotops..??????

2 2147

what is gram staining

Shantha Biotech,

4 4903

what is the reducing product when barfoed's reagent react with a monosaccharide?

2 1905




If we removed spleen from the body,what happens?is there any disturbance to immune system?

3 2916

what is meant by codan optimisation m rna enrichment?

CSIR,

1279

which is more important, TCA or glycolysis??

6 3460

if there are ester and aldehyde substituents on the pyridine ring . how I can oxidized the aldehyde substituent to carboxylic acid ? please help me

1 1665

find the name of 4 such rivers which form delta?

GV,

3 11820




why protein was considered as genitic material than DNA

3 3094

why DNA is not directly trsnslated into protein rather than it is using RNA as intermediate?

4 5927

HOW THE ELECTRONS AND PROTONS ,NEUTRONS WHER FOUND INSIDE THE ELEMENT,AND HOW THEY PROOVED THAT ELECTRONS ARE ROTATING AROUND THE NEUTRONS AND PROTONS

Vimta Labs,

1 1946

you have a mixture of dNTPs(deoxynucleotide triphosphates)in a sample.how can you separate these all?

IISc,

3 3885

why life span of RBC IS GREATER THEN THE WBC?

1343

why we use 96 well plate in ELISA plate reader?

1065

Cloning is a process where the nucleus of one egg is taken and it's coated with the membrane of other individual, to get a duplicate of a person. so my question is:when we use egg of some individual and membrane of some other how will we get a duplicate of a particular individual?

3 1933


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Un-Answered Questions { Bio Chemistry }

How much empty space is found in globular proteins?

838


How are free energy changes related to LeChatelier's Principle?

738


How are reaction rates dependent upon free energy changes?

900


can i used antimony potassium tartrate against potassium tartrate in Fehling B solution ?

449


Why does the pH of the blood decrease in a person who has digested trematol?

1010






why ph is not more than 14?

183


What type of column is generally used to separate amino acids from each other?

927


How does hplc differ from normal column chromatography, and what are its advantages?

886


I understand that urine can act as a reagent to break disulfide bonds? I am particularly interested in bonds between cysteines. and or any other amino acids.

190


What do you like LEAST & MOST about your current position?

3030


Describe the structure of a peptide giving the sequence.

668


A 3.00 * 10-6M solution in a 1.0 cm cuvette read 16 % T at 620nm. What were the absorbance and the molar absorbancy index of the solution?

854


I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line

866


Two children have a neurological disorder. When cells from the two patients were fused to form heterokaryons containing nuclei from both patients and cultured, the cells displayed normal metabolism. Cells from either patient exhibited abnormal metabolism when cultured separately.what does these results indicate?

905


When it functions as a "second messenger", cAMP a) acts outside the cell to influence cellular processes. b) acts "second in importance" to AMP. c) activates all cytosolic protein kinases. d) activates the cAMP-dependent protein kinase.

854