Bio Chemistry Interview Questions
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What factors contribute to the enthalpy change when a denatured protein folds up?

1 1742

What role does proline play in tertiary structures?

1 2087

What types of changes can be used to effect fractional precipitation of proteins?

3 2710

What reaction is catalyzed by proteases? What steps are involved in determining the primary structure of a protein?

2 18843

What are the differences and respective advantages to electrophoresis, SDS PAGE, and isoelectric focusing?

6 18498

What features do the helix and the sheet have in common?

4 2652

What are the structures of ATP, ADP, PPi and Pi?

2 15776

Describe the classification of all amino acids .

4 5873

What reagents can be used to break disulfide bonds?

10 20496

What is the major advantage of Edman’s reagent?

4 10725

Where does ATP rank on a scale of phosphate transfer potential? What is the significance of this location?

3 4758

How do you measure the pollution in water

4 3672

How to measure average peptide chain length

2 16811

I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line


bane the by products when crude oil is refined by distillation


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Un-Answered Questions { Bio Chemistry }

During a heart attack, blood flowing to the heart muscle is interrupted by blockage of a coronary artery. How would you expect the metabolism in the heart to change?


How does a random coil differ from an irregularly structured region?


Why does physical exertion increase symptoms of poisoning by trematol?


which fat soluble vitamin has co-enzymic function?


What factors can change the pKa value of an amino acid residue, and in which direction?


I understand that urine can act as a reagent to break disulfide bonds? I am particularly interested in bonds between cysteines. and or any other amino acids.


Which organ and subcellular site are most important for fatty acid biosynthesis?


bane the by products when crude oil is refined by distillation


How does hplc differ from normal column chromatography, and what are its advantages?


How do peptides react with cyanogen bromide?


How are coupled reactions used in biochemistry?


"Tell me about a job that you found particularly satisfying. What did you like about it?


Which vitamins participate, in coenzyme form, in reactions of the tricarboxylic acid cycle?


What is the difference between Phospholipids and phosphosphingosides?


why we use 96 well plate in ELISA plate reader?