What factors contribute to the enthalpy change when a denatured protein folds up?1 1821
What role does proline play in tertiary structures?1 2188
What types of changes can be used to effect fractional precipitation of proteins?3 2808
What reaction is catalyzed by proteases? What steps are involved in determining the primary structure of a protein?2 19565
What are the differences and respective advantages to electrophoresis, SDS PAGE, and isoelectric focusing?6 19095
What features do the helix and the sheet have in common?4 2789
What are the structures of ATP, ADP, PPi and Pi?2 17258
Describe the classification of all amino acids .4 5980
What reagents can be used to break disulfide bonds?10 21627
What is the major advantage of Edman’s reagent?4 11683
Where does ATP rank on a scale of phosphate transfer potential? What is the significance of this location?3 4878
How do you measure the pollution in water4 3837
How to measure average peptide chain length2 17267
I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line914
what is your aspiration in profession/ career?
how I cell disease is caused?
what is familiar hypercholesterolemia?
Which compound is involved in reducing levels of homocysteine in the blood?
What is the difference between Phospholipids and phosphosphingosides?
What do you like LEAST & MOST about your current position?
What are the drawbacks of tissue slices technique?
What factors can change the pKa value of an amino acid residue, and in which direction?
Why are free cysteines treated with iodoacetate prior to protein sequencing?
Why is the t-butyloxycarbonyl protecting group such a good choice for amino acid synthesis?
All technical,written test questions
What type of column is generally used to separate amino acids from each other?
During a heart attack, blood flowing to the heart muscle is interrupted by blockage of a coronary artery. How would you expect the metabolism in the heart to change?
Calculate the molar extinction coefficient of a solution containing 5 *10-4 g litre-1 of a biomolecule, molecular weight 275 g mol-1, and absorbance 0.75 in a 1.2 cm cuvette.
what is your passion?