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What reaction is catalyzed by proteases? What steps are involved in determining the primary structure of a protein?
2 23078What are the differences and respective advantages to electrophoresis, SDS PAGE, and isoelectric focusing?
6 25380Where does ATP rank on a scale of phosphate transfer potential? What is the significance of this location?
3 8363I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line
2236Post New Bio Chemistry Questions
What is dipole?
What are the main factors that alter the speed of enzymatic reactions?
The expression q=delta (e)-w is? What does it states?
How many elements are there in 3d-series of first transition series?
How does the substrate concentration affect the speed of enzymatic reactions?
Diborane contains how many banana bonds?
Into which two groups can the nitrogen-containing bases that form dna and rna be classified? What is the criterion used in that classification?
What is the anhydride of n2o5?
What does one Debye equals?
Aldehydes (ch3cho) and vinyl alcohol (ch2=choh) are?
Explain and define standard feat of formation
In electronic conductors, what is the reason for flow of current?
What is ph? What are the units of ph?
What is the completing sequence of nitrogen-containing bases for an agccgttaac fragment of a dna chain?
Why is the t-butyloxycarbonyl protecting group such a good choice for amino acid synthesis?
