Bio Chemistry Interview Questions
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What factors contribute to the enthalpy change when a denatured protein folds up?

1 1917

What role does proline play in tertiary structures?

1 2288

What types of changes can be used to effect fractional precipitation of proteins?

3 2927




What reaction is catalyzed by proteases? What steps are involved in determining the primary structure of a protein?

2 19998

What are the differences and respective advantages to electrophoresis, SDS PAGE, and isoelectric focusing?

6 19577

What features do the helix and the sheet have in common?

4 2922

What are the structures of ATP, ADP, PPi and Pi?

2 17999

Describe the classification of all amino acids .

4 6084




What reagents can be used to break disulfide bonds?

10 22370

What is the major advantage of Edman’s reagent?

4 12133

Where does ATP rank on a scale of phosphate transfer potential? What is the significance of this location?

3 5034

How do you measure the pollution in water

4 3983

How to measure average peptide chain length

2 17708

I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line

996

bane the by products when crude oil is refined by distillation

1041


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Un-Answered Questions { Bio Chemistry }

Which vitamins participate, in coenzyme form, in reactions of the tricarboxylic acid cycle?

1006


DNA binding by proteins with the helix-turn-helix (HTH) motif does not involve a) altered stacking of the DNA at the center of symmetry. b) hydrogen bonds, salt bridges, and van der Waals contacts. c) interactions with base pairs in the major groove of DNA. d) interactions with the sugar-phosphate backbone of DNA.

967


what is QC and why is impotent in pathology lab

1609


How are free energy changes related to enthalpy changes and entropy changes?

904


does glucagon secretion induce sleep in teenagers?why?

1089






What are the drawbacks of tissue slices technique?

1073


How does hplc differ from normal column chromatography, and what are its advantages?

1027


why life span of RBC IS GREATER THEN THE WBC?

1459


How does a random coil differ from an irregularly structured region?

1059


What are the structures of the products of this reaction, and how are they identified?

971


Describe the structure of a peptide giving the sequence.

768


What are hydrophobicity scales, and how are they used?

828


The elution volume of an enzyme on a gel filtration column can be predicted from a) its enzyme activity b) its protein absorbance at 280 nm c) subunit composition and monomer molecular mass(es) d) Choices a) and b) are both correct.

2024


When it functions as a "second messenger", cAMP a) acts outside the cell to influence cellular processes. b) acts "second in importance" to AMP. c) activates all cytosolic protein kinases. d) activates the cAMP-dependent protein kinase.

954


In which forms arsenic impurities present in drug.

173