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Bio Chemistry Interview Questions
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What factors contribute to the enthalpy change when a denatured protein folds up?

1 2312

What role does proline play in tertiary structures?

1 2705

What types of changes can be used to effect fractional precipitation of proteins?

3 3488

What reaction is catalyzed by proteases? What steps are involved in determining the primary structure of a protein?

2 20848

What are the differences and respective advantages to electrophoresis, SDS PAGE, and isoelectric focusing?

6 21453

What features do the helix and the sheet have in common?

4 3866

What are the structures of ATP, ADP, PPi and Pi?

2 19624

Describe the classification of all amino acids .

4 6851

What reagents can be used to break disulfide bonds?

10 25239

What is the major advantage of Edman’s reagent?

4 13469

Where does ATP rank on a scale of phosphate transfer potential? What is the significance of this location?

3 5812

How do you measure the pollution in water

4 4845

How to measure average peptide chain length

2 19185

I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line

1336

bane the by products when crude oil is refined by distillation

1321


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Un-Answered Questions { Bio Chemistry }

Do enzymes act better under acid or basic ph?

253


What are zymogens?

235


What are the units of bond length?

255


What is the structure of cscl and give the co-ordination number of Cscl?

243


In electronic conductors, what is the reason for flow of current?

223






Which metal is more metallic in nature among 1A group elements?

225


Two children have a neurological disorder. When cells from the two patients were fused to form heterokaryons containing nuclei from both patients and cultured, the cells displayed normal metabolism. Cells from either patient exhibited abnormal metabolism when cultured separately.what does these results indicate?

1302


Why ethylene undergoes electrophilic addition reactions?

221


What is xylose?

223


What is the main enzyme that participates in dna duplication process?

205


Why fe3+ ion is more stable than fe2+ ion is?

251


What factors can change the pKa value of an amino acid residue, and in which direction?

1692


What does one Debye equals?

261


what is familiar hypercholesterolemia?

1595


What are the structures of the products of this reaction, and how are they identified?

1213