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What reaction is catalyzed by proteases? What steps are involved in determining the primary structure of a protein?
2 22916What are the differences and respective advantages to electrophoresis, SDS PAGE, and isoelectric focusing?
6 25133Where does ATP rank on a scale of phosphate transfer potential? What is the significance of this location?
3 8171I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line
2153Post New Bio Chemistry Questions
Why does physical exertion increase symptoms of poisoning by trematol?
What is structure of Nacl and give the co-ordination number of Nacl?
Aldehydes and ketones are known as?
Define polar covalent bond?
how I cell disease is caused?
What is electrophilic addition reaction?
why life span of RBC IS GREATER THEN THE WBC?
Into which two groups can the nitrogen-containing bases that form dna and rna be classified? What is the criterion used in that classification?
What is the enzyme that catalyzes the process for production of rna?
What is the rule for the pairing of nitrogen-containing bases in the dna molecule and in the rna?
What is meant by substrates of enzymatic reactions?
What happens in methylation of dna?
The expression q=delta (e)-w is? What does it states?
What does hess law states?
Give the example for electrophilic substitution reaction?
