What factors contribute to the enthalpy change when a denatured protein folds up?1 1796
What role does proline play in tertiary structures?1 2140
What types of changes can be used to effect fractional precipitation of proteins?3 2777
What reaction is catalyzed by proteases? What steps are involved in determining the primary structure of a protein?2 19363
What are the differences and respective advantages to electrophoresis, SDS PAGE, and isoelectric focusing?6 18920
What features do the helix and the sheet have in common?4 2745
What are the structures of ATP, ADP, PPi and Pi?2 16917
Describe the classification of all amino acids .4 5941
What reagents can be used to break disulfide bonds?10 21292
What is the major advantage of Edman’s reagent?4 11467
Where does ATP rank on a scale of phosphate transfer potential? What is the significance of this location?3 4820
How do you measure the pollution in water4 3787
How to measure average peptide chain length2 17118
I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line889
How are reaction rates dependent upon free energy changes?
Calculate the molar extinction coefficient of a solution containing 5 *10-4 g litre-1 of a biomolecule, molecular weight 275 g mol-1, and absorbance 0.75 in a 1.2 cm cuvette.
Glycolysis is A) C6H12O6 --> 2C2H5OH + 2CO2 B) C6H12O6 --> 2CH3H4O3 + 4H c) C3H4O3 + NAD --> C2H5OH + CO2 NAD D) C6H12O6 + 6H20 --> 6CO2 + 6H2O
All technical,written test questions
Why are right-handed helices more stable than left-handed helices?
Two children have a neurological disorder. When cells from the two patients were fused to form heterokaryons containing nuclei from both patients and cultured, the cells displayed normal metabolism. Cells from either patient exhibited abnormal metabolism when cultured separately.what does these results indicate?
DNA binding by proteins with the helix-turn-helix (HTH) motif does not involve a) altered stacking of the DNA at the center of symmetry. b) hydrogen bonds, salt bridges, and van der Waals contacts. c) interactions with base pairs in the major groove of DNA. d) interactions with the sugar-phosphate backbone of DNA.
Why is the t-butyloxycarbonyl protecting group such a good choice for amino acid synthesis?
Which vitamins participate, in coenzyme form, in reactions of the tricarboxylic acid cycle?
Hi, I am venkat. I am done with M.Sc Medical Biochemistry in the year 2007. I got rejected 3 times at US consulate, and the reason they say is why are you going agian for PG when you have already done PG, and they are not ready to get convinced only on this issue.
Describe the structure of a peptide giving the sequence.
How are coupled reactions used in biochemistry?
How are free energy, equilibrium and spontaneity related to each other?
what are the steps involved in the calibration of HPLC
why life span of RBC IS GREATER THEN THE WBC?