What factors contribute to the enthalpy change when a denatured protein folds up?1 2312
What role does proline play in tertiary structures?1 2705
What types of changes can be used to effect fractional precipitation of proteins?3 3488
What reaction is catalyzed by proteases? What steps are involved in determining the primary structure of a protein?2 20848
What are the differences and respective advantages to electrophoresis, SDS PAGE, and isoelectric focusing?6 21453
What features do the helix and the sheet have in common?4 3866
What are the structures of ATP, ADP, PPi and Pi?2 19624
Describe the classification of all amino acids .4 6851
What reagents can be used to break disulfide bonds?10 25239
What is the major advantage of Edman’s reagent?4 13469
Where does ATP rank on a scale of phosphate transfer potential? What is the significance of this location?3 5812
How do you measure the pollution in water4 4845
How to measure average peptide chain length2 19185
I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line1336
Do enzymes act better under acid or basic ph?
What are zymogens?
What are the units of bond length?
What is the structure of cscl and give the co-ordination number of Cscl?
In electronic conductors, what is the reason for flow of current?
Which metal is more metallic in nature among 1A group elements?
Two children have a neurological disorder. When cells from the two patients were fused to form heterokaryons containing nuclei from both patients and cultured, the cells displayed normal metabolism. Cells from either patient exhibited abnormal metabolism when cultured separately.what does these results indicate?
Why ethylene undergoes electrophilic addition reactions?
What is xylose?
What is the main enzyme that participates in dna duplication process?
Why fe3+ ion is more stable than fe2+ ion is?
What factors can change the pKa value of an amino acid residue, and in which direction?
What does one Debye equals?
what is familiar hypercholesterolemia?
What are the structures of the products of this reaction, and how are they identified?