Bio Chemistry Interview Questions
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What factors contribute to the enthalpy change when a denatured protein folds up?

1 1866

What role does proline play in tertiary structures?

1 2242

What types of changes can be used to effect fractional precipitation of proteins?

3 2877

What reaction is catalyzed by proteases? What steps are involved in determining the primary structure of a protein?

2 19813

What are the differences and respective advantages to electrophoresis, SDS PAGE, and isoelectric focusing?

6 19330

What features do the helix and the sheet have in common?

4 2858

What are the structures of ATP, ADP, PPi and Pi?

2 17660

Describe the classification of all amino acids .

4 6034

What reagents can be used to break disulfide bonds?

10 22011

What is the major advantage of Edman’s reagent?

4 11961

Where does ATP rank on a scale of phosphate transfer potential? What is the significance of this location?

3 4959

How do you measure the pollution in water

4 3917

How to measure average peptide chain length

2 17499

I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line


bane the by products when crude oil is refined by distillation


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Un-Answered Questions { Bio Chemistry }

How do hydrophobic and hydrophilic residues arrange themselves in globular proteins?


Why does physical exertion increase symptoms of poisoning by trematol?


What is zone electrophoresis?


Why are free cysteines treated with iodoacetate prior to protein sequencing?


How are free energy changes related to LeChatelier's Principle?


bane the by products when crude oil is refined by distillation


Glycolysis is A) C6H12O6 --> 2C2H5OH + 2CO2 B) C6H12O6 --> 2CH3H4O3 + 4H c) C3H4O3 + NAD --> C2H5OH + CO2 NAD D) C6H12O6 + 6H20 --> 6CO2 + 6H2O


How are free energy changes related to enthalpy changes and entropy changes?


Why is the t-butyloxycarbonyl protecting group such a good choice for amino acid synthesis?


The elution volume of an enzyme on a gel filtration column can be predicted from a) its enzyme activity b) its protein absorbance at 280 nm c) subunit composition and monomer molecular mass(es) d) Choices a) and b) are both correct.


What factors are involved in determining the electrophoretic mobility of a molecule?


Calculate the molar extinction coefficient of a solution containing 5 *10-4 g litre-1 of a biomolecule, molecular weight 275 g mol-1, and absorbance 0.75 in a 1.2 cm cuvette.


Describe the environment where you do your best work in an organization?


How much empty space is found in globular proteins?


How does hplc differ from normal column chromatography, and what are its advantages?