What factors contribute to the enthalpy change when a denatured protein folds up?1 1768
What role does proline play in tertiary structures?1 2117
What types of changes can be used to effect fractional precipitation of proteins?3 2747
What reaction is catalyzed by proteases? What steps are involved in determining the primary structure of a protein?2 19185
What are the differences and respective advantages to electrophoresis, SDS PAGE, and isoelectric focusing?6 18766
What features do the helix and the sheet have in common?4 2706
What are the structures of ATP, ADP, PPi and Pi?2 16593
Describe the classification of all amino acids .4 5914
What reagents can be used to break disulfide bonds?10 20960
What is the major advantage of Edman’s reagent?4 11203
Where does ATP rank on a scale of phosphate transfer potential? What is the significance of this location?3 4795
How do you measure the pollution in water4 3735
How to measure average peptide chain length2 16999
I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line867
How does a random coil differ from an irregularly structured region?
What are hydrophobicity scales, and how are they used?
what are the steps involved in the calibration of HPLC
What is the structural formula of molybdenum blue?
bane the by products when crude oil is refined by distillation
what is QC and why is impotent in pathology lab
Describe the structure of a peptide giving the sequence.
i have done M.SC. in biochemistry from india. as i have experience of pharmaceuticals in quality assurance department of 4 year.now i come to know that my degree is valid in uk.i got my comparision letter of degree from uknaric. but i m confuse that what is the next step to do for getiing job in lab? am i eligible to get directly job or as trainee than job ? or to register in ibms or what? pls tell me
The elution volume of an enzyme on a gel filtration column can be predicted from a) its enzyme activity b) its protein absorbance at 280 nm c) subunit composition and monomer molecular mass(es) d) Choices a) and b) are both correct.
How are free energy changes related to LeChatelier's Principle?
Which organ and subcellular site are most important for fatty acid biosynthesis?
What are the drawbacks of tissue slices technique?
How are coupled reactions used in biochemistry?
what is your passion?
What is the difference between Phospholipids and phosphosphingosides?