Bio Chemistry Interview Questions
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What factors contribute to the enthalpy change when a denatured protein folds up?

1 1821

What role does proline play in tertiary structures?

1 2188

What types of changes can be used to effect fractional precipitation of proteins?

3 2808




What reaction is catalyzed by proteases? What steps are involved in determining the primary structure of a protein?

2 19565

What are the differences and respective advantages to electrophoresis, SDS PAGE, and isoelectric focusing?

6 19095

What features do the helix and the sheet have in common?

4 2789

What are the structures of ATP, ADP, PPi and Pi?

2 17258

Describe the classification of all amino acids .

4 5980




What reagents can be used to break disulfide bonds?

10 21627

What is the major advantage of Edman’s reagent?

4 11683

Where does ATP rank on a scale of phosphate transfer potential? What is the significance of this location?

3 4878

How do you measure the pollution in water

4 3837

How to measure average peptide chain length

2 17267

I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line

914

bane the by products when crude oil is refined by distillation

976


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Un-Answered Questions { Bio Chemistry }

what is your aspiration in profession/ career?

1518


how I cell disease is caused?

1203


what is familiar hypercholesterolemia?

1221


Which compound is involved in reducing levels of homocysteine in the blood?

1080


What is the difference between Phospholipids and phosphosphingosides?

1923






What do you like LEAST & MOST about your current position?

3074


What are the drawbacks of tissue slices technique?

1011


What factors can change the pKa value of an amino acid residue, and in which direction?

1312


Why are free cysteines treated with iodoacetate prior to protein sequencing?

1069


Why is the t-butyloxycarbonyl protecting group such a good choice for amino acid synthesis?

963


All technical,written test questions

438


What type of column is generally used to separate amino acids from each other?

960


During a heart attack, blood flowing to the heart muscle is interrupted by blockage of a coronary artery. How would you expect the metabolism in the heart to change?

1734


Calculate the molar extinction coefficient of a solution containing 5 *10-4 g litre-1 of a biomolecule, molecular weight 275 g mol-1, and absorbance 0.75 in a 1.2 cm cuvette.

1963


what is your passion?

840