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What are the differences and respective advantages to
electrophoresis, SDS PAGE, and isoelectric focusing?
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Answer / g.rajesh kumar
Electrophoresis is a separation technique especially used
for seperation of protein molecules.
Eletrophoresis involves the use of electricity which makes
the molecules to migrate based on their charge.
IEF separates sample compounds(proteins) according to
isoelectric point,
whereas SDS-PAGE separates the compounds by molecular weight.
| Is This Answer Correct ? | 28 Yes | 6 No |
Answer / moumita ghosal
electrophoresis helps in separation and purification of
protein based on charge: mass ratio property.
SDS-PAGE helps in molecular weight determination. also it
allows to know how many subunit the protein has.
Isoelectric focusing helps in separation and precipitation
of protein at their isoelectric point(net charge is zero).
| Is This Answer Correct ? | 15 Yes | 3 No |
Answer / surbhi
SDS PAGE is widely used method for analysing protein
mixtures qualitatively.it is particularly useful for
monitoring protein purification on the basis of their size.
isoelectric focusing method is ideal for separtion of
amphoteric ssubstances such as proteins because it is based
on the separation of molecules according to their different
isoelectric points.
| Is This Answer Correct ? | 6 Yes | 0 No |
Answer / b.adhikary
Electrophoresis is a separation technique especially used
for seperation of protein molecules.
IEF separates sample compounds(proteins) according to
isoelectric point,
SDS-PAGE separates the compounds by molecular weight and
there ph (charge).
| Is This Answer Correct ? | 1 Yes | 1 No |
Answer / khushboo raheja
SDS (LAURYL) used for electrophoresis and general cell lysis
application.
SDS save time,save cost,save trouble.
| Is This Answer Correct ? | 0 Yes | 4 No |
Answer / subbareddy1984
electrophoresis is the migraion of charged particals or ions
by under the influence of an applied electic field.
separation is mainly based on charge and mass.
SDS-PAGE is used for the molecularweight determinations of
ions or molecules like proteins .
isoelecticfocusing is used for the seperation of charged
sparticals and mixture of proteins(aminoacids).
| Is This Answer Correct ? | 1 Yes | 7 No |
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