Bio Chemistry Interview Questions
Questions Answers Views Company eMail

Why is it necessary to cleave proteins with at least two different reagents during sequencing?

1 1543

How are coupled reactions used in biochemistry?

1122

Cells are in nonequilibrium, open systems. In what way is thermodynamics useful in this situation?

1 1678




What type of column is generally used to separate amino acids from each other?

960

Which properties of water are unusual for its size, and caused by hydrogen bonding?

1 1451

What kinds of proteins are involved in facilitating protein folding?

1 1918

What types of molecules can be purified via affinity chromatography?

1 1889

How can proteins be denatured and renatured?

1 2702




What does a sedimentation coefficient measure?

1 1914

How flexible are protein structures?

1 1571

What causes aromatic rings to stick to each other?

1 1648

How does H-bonding in the helix differ from that in the sheet?

1 1315

Why is the t-butyloxycarbonyl protecting group such a good choice for amino acid synthesis?

963

Why don’t all proteins have methionine as the N-terminal amino acid?

2 3473

How much empty space is found in globular proteins?

Bharat Biotech,

882


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Un-Answered Questions { Bio Chemistry }

what is your aspiration in profession/ career?

1519


What are the drawbacks of tissue slices technique?

1011


How are free energy, equilibrium and spontaneity related to each other?

962


does glucagon secretion induce sleep in teenagers?why?

1013


can i used antimony potassium tartrate against potassium tartrate in Fehling B solution ?

482






What do you like LEAST & MOST about your current position?

3074


What are species grow in waitland?

1218


why ph is not more than 14?

236


Which organ and subcellular site are most important for fatty acid biosynthesis?

997


The nucleophile in the first stage of the serine protease mechanism is a) H2O b) the carboxyl of Asp 102 c) the hydroxyl of Ser 195 d) the imidazole of His 57

947


DNA binding by proteins with the helix-turn-helix (HTH) motif does not involve a) altered stacking of the DNA at the center of symmetry. b) hydrogen bonds, salt bridges, and van der Waals contacts. c) interactions with base pairs in the major groove of DNA. d) interactions with the sugar-phosphate backbone of DNA.

899


bane the by products when crude oil is refined by distillation

976


I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line

914


i have done M.SC. in biochemistry from india. as i have experience of pharmaceuticals in quality assurance department of 4 year.now i come to know that my degree is valid in uk.i got my comparision letter of degree from uknaric. but i m confuse that what is the next step to do for getiing job in lab? am i eligible to get directly job or as trainee than job ? or to register in ibms or what? pls tell me

2131


what is familiar hypercholesterolemia?

1221