What reagents can be used to break disulfide bonds?
Answers were Sorted based on User's Feedback
Answer / ravindra naik
|Is This Answer Correct ?||53 Yes||2 No|
Answer / raghavendra naidu
1)DTT- Di thio tritol
2)BME- Beta mercapto ethanol
|Is This Answer Correct ?||38 Yes||0 No|
Answer / g.rajesh kumar
Mercaptoethanol breaks the disulphide bonds in proteins and
sds makes the protein to possess negative charge, so that
proteins can be separated based on their charge in sds-page
|Is This Answer Correct ?||23 Yes||1 No|
Answer / s
|Is This Answer Correct ?||22 Yes||1 No|
Answer / nano
|Is This Answer Correct ?||11 Yes||1 No|
Answer / narayana
Di thio thretol,Beta marcapto ethanol.
Some times urine also.
|Is This Answer Correct ?||2 Yes||0 No|
Answer / g satheesh
sodium bisulfite in Base media or Zinc and acetic acid
|Is This Answer Correct ?||3 Yes||2 No|
Answer / madhukar
|Is This Answer Correct ?||2 Yes||4 No|
Answer / firstname.lastname@example.org
SDS pocess the proteins to positive charge and b-mercato ethanol degrades the disulphide bonds. and urea also degrades the disulphide bonds
|Is This Answer Correct ?||1 Yes||4 No|
Answer / guest
|Is This Answer Correct ?||10 Yes||45 No|
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