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What reagents can be used to break disulfide bonds?

Answers were Sorted based on User's Feedback



What reagents can be used to break disulfide bonds?..

Answer / ravindra naik

mercapto ethanol

Is This Answer Correct ?    53 Yes 2 No

What reagents can be used to break disulfide bonds?..

Answer / raghavendra naidu

1)DTT- Di thio tritol
2)BME- Beta mercapto ethanol

Is This Answer Correct ?    38 Yes 0 No

What reagents can be used to break disulfide bonds?..

Answer / g.rajesh kumar

Mercaptoethanol breaks the disulphide bonds in proteins and
sds makes the protein to possess negative charge, so that
proteins can be separated based on their charge in sds-page
chromatography.

Is This Answer Correct ?    23 Yes 1 No

What reagents can be used to break disulfide bonds?..

Answer / s

beta-mercaptoethanol, dithiothreitol

Is This Answer Correct ?    22 Yes 1 No

What reagents can be used to break disulfide bonds?..

Answer / nano

1-DTT
2-TCEP

Is This Answer Correct ?    11 Yes 1 No

What reagents can be used to break disulfide bonds?..

Answer / narayana

Di thio thretol,Beta marcapto ethanol.
Some times urine also.

Is This Answer Correct ?    2 Yes 0 No

What reagents can be used to break disulfide bonds?..

Answer / g satheesh

sodium bisulfite in Base media or Zinc and acetic acid

Is This Answer Correct ?    3 Yes 2 No

What reagents can be used to break disulfide bonds?..

Answer / madhukar

sds b-mercaptoethanol

Is This Answer Correct ?    2 Yes 4 No

What reagents can be used to break disulfide bonds?..

Answer / kesava.sat@gmail.com

SDS pocess the proteins to positive charge and b-mercato ethanol degrades the disulphide bonds. and urea also degrades the disulphide bonds

Is This Answer Correct ?    1 Yes 4 No

What reagents can be used to break disulfide bonds?..

Answer / guest

SDS

Is This Answer Correct ?    10 Yes 45 No

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