Bio Chemistry Interview Questions
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THE SMALL CARTILAGE OF OUR BODY IS.....

1 1994

what is the wood sugar?

1 1977

Calculate the molar extinction coefficient of a solution containing 5 *10-4 g litre-1 of a biomolecule, molecular weight 275 g mol-1, and absorbance 0.75 in a 1.2 cm cuvette.

1963




A 3.00 * 10-6M solution in a 1.0 cm cuvette read 16 % T at 620nm. What were the absorbance and the molar absorbancy index of the solution?

887

what is the difference between hydrolase, synthase and synthetase

1 10236

what are the disadvantages of immobilized enzymes

2 12467

Hi, I am venkat. I am done with M.Sc Medical Biochemistry in the year 2007. I got rejected 3 times at US consulate, and the reason they say is why are you going agian for PG when you have already done PG, and they are not ready to get convinced only on this issue.

951

how the d-amino acids are converted into L- AMINO ACIDS?

IIT,

3 2634




what is your aspiration in profession/ career?

1518

what are you really good at?

2 1759

what is your passion?

840

"Tell me about a job that you found particularly satisfying. What did you like about it?

1872

What do you like LEAST & MOST about your current position?

3074

Describe the environment where you do your best work in an organization?

953

blood glucose is originated from??

BioChem,

3 3015


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Un-Answered Questions { Bio Chemistry }

How are free energy changes related to LeChatelier's Principle?

794


How are free energy, equilibrium and spontaneity related to each other?

962


what is meant by codan optimisation m rna enrichment?

1327


How do hydrophobic and hydrophilic residues arrange themselves in globular proteins?

837


what is familiar hypercholesterolemia?

1221






Why does physical exertion increase symptoms of poisoning by trematol?

1509


Which vitamins participate, in coenzyme form, in reactions of the tricarboxylic acid cycle?

918


Which organ and subcellular site are most important for fatty acid biosynthesis?

804


why we use 96 well plate in ELISA plate reader?

1118


I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line

913


What factors are involved in determining the electrophoretic mobility of a molecule?

906


How is the actual free energy change for a process related to the standard free energy change?

1480


What type of column is generally used to separate amino acids from each other?

960


Why does the pH of the blood decrease in a person who has digested trematol?

1066


How much empty space is found in globular proteins?

882