Bio Chemistry Interview Questions
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THE SMALL CARTILAGE OF OUR BODY IS.....

1 2014

what is the wood sugar?

1 2001

Calculate the molar extinction coefficient of a solution containing 5 *10-4 g litre-1 of a biomolecule, molecular weight 275 g mol-1, and absorbance 0.75 in a 1.2 cm cuvette.

1975




A 3.00 * 10-6M solution in a 1.0 cm cuvette read 16 % T at 620nm. What were the absorbance and the molar absorbancy index of the solution?

900

what is the difference between hydrolase, synthase and synthetase

1 10295

what are the disadvantages of immobilized enzymes

2 12556

Hi, I am venkat. I am done with M.Sc Medical Biochemistry in the year 2007. I got rejected 3 times at US consulate, and the reason they say is why are you going agian for PG when you have already done PG, and they are not ready to get convinced only on this issue.

965

how the d-amino acids are converted into L- AMINO ACIDS?

IIT,

3 2667




what is your aspiration in profession/ career?

1543

what are you really good at?

2 1783

what is your passion?

857

"Tell me about a job that you found particularly satisfying. What did you like about it?

1895

What do you like LEAST & MOST about your current position?

3098

Describe the environment where you do your best work in an organization?

966

blood glucose is originated from??

BioChem,

3 3041


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Un-Answered Questions { Bio Chemistry }

How does a random coil differ from an irregularly structured region?

998


The elution volume of an enzyme on a gel filtration column can be predicted from a) its enzyme activity b) its protein absorbance at 280 nm c) subunit composition and monomer molecular mass(es) d) Choices a) and b) are both correct.

1937


How is the actual free energy change for a process related to the standard free energy change?

1499


Why are free cysteines treated with iodoacetate prior to protein sequencing?

1085


Which compound is involved in reducing levels of homocysteine in the blood?

1090






Two children have a neurological disorder. When cells from the two patients were fused to form heterokaryons containing nuclei from both patients and cultured, the cells displayed normal metabolism. Cells from either patient exhibited abnormal metabolism when cultured separately.what does these results indicate?

968


DNA binding by proteins with the helix-turn-helix (HTH) motif does not involve a) altered stacking of the DNA at the center of symmetry. b) hydrogen bonds, salt bridges, and van der Waals contacts. c) interactions with base pairs in the major groove of DNA. d) interactions with the sugar-phosphate backbone of DNA.

913


What type of column is generally used to separate amino acids from each other?

971


bane the by products when crude oil is refined by distillation

989


Describe the structure of a peptide giving the sequence.

724


Which vitamins participate, in coenzyme form, in reactions of the tricarboxylic acid cycle?

933


why ph is not more than 14?

249


How are reaction rates dependent upon free energy changes?

1016


I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line

926


can i used antimony potassium tartrate against potassium tartrate in Fehling B solution ?

494